Biomineralization Directed by Prenucleated Calcium and Phosphorus Nanoclusters Improving Mechanical Properties and Osteogenic Potential of Antheraea pernyi Silk Fibroin-Based Artificial Periosteum
Biomineralization Directed by Prenucleated Calcium and Phosphorus Nanoclusters Improving Mechanical Properties and Osteogenic Potential of Antheraea pernyi Silk Fibroin-Based Artificial Periosteum
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预成核钙磷纳米团簇引导生物矿化改善柞蚕丝素蛋白基人工骨膜的机械性能和成骨潜力
DOI：
10.1002/adhm.202001695
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发表时间：
2021
影响因子：
10
通讯作者：
Yang Mingying
中科院分区：
文献类型：
--
作者：
Shuai Yajun;Lu Huan;Lv Ruyin;Wang Jie;Wan Quan;Mao Chuanbin;Yang Mingying
利用生物大分子作为模板控制羟基磷灰石晶体的成核和生长来制备生物活性材料是骨组织工程中一条有价值的途径。本研究采用生物矿化的方法制备了一种人工骨膜，该人工骨膜可促进骨髓间充质干细胞(MSCs)的成骨分化，并在体内诱导骨基质蛋白的形成。为了实现这一目标，设计了一种受生物启发的预核钙磷纳米团簇矿化系统，以在AF膜表面成核并生成羟基磷灰石晶体。这种生物矿化过程为AF膜提供了更好的弹性模数和拉伸强度。细胞存活率、溶血试验和H&E染色结果表明，矿化纤维(MAF)膜具有良好的细胞相容性、血液相容性和体内外组织相容性。此外，在没有成骨诱导剂的情况下，MAF膜在体外可显著促进MSCs的成骨分化。体内实验表明，无论有无骨髓间充质干细胞接种，骨髓基质细胞组均高表达骨相关基质蛋白。因此，在生物矿化AF膜的基础上，利用生物启发的前核纳米簇来制备人工骨膜是骨组织工程领域的一种很有前途的策略。
The use of biomacromolecules as templates to control the nucleation and growth of hydroxyapatite crystals to prepare bioactive materials is a valuable approach in bone tissue engineering. Here, an artificial periosteum is prepared by biomineralizingAntheraea pernyifibroin (AF) membrane with prenucleated nanoclusters, which can promote the osteogenic differentiation of mesenchymal stem cells (MSCs) and induce the formation of bone matrix protein in vivo. To achieve this, a biologically inspired prenucleated calcium and phosphorus nanocluster mineralization system is designed to nucleate and generate hydroxyapatite crystals on the surface of theAFmembrane. This biomineralization process providesAFmembranes with improved elastic modulus and tensile strength. Subsequently, cell viability assay, hemolysis test, and H&E staining show that the mineralizedAF(MAF) membranes has good cytocompatibility, hemocompatibility, and histocompatibility in vitro and in vivo. Additionally, theMAFmembranes significantly promote osteogenic differentiation of MSCs in the absence of osteogenic inducer in vitro. Experiments in vivo demonstrate that bone‐related matrix proteins are highly expressed inMAFgroups with or without MSCs seeded. Therefore, the use of bioinspired prenucleated nanoclusters to prepare artificial periosteum based on biomineralizedAFmembrane is a promising strategy in the field of bone tissue engineering.