Granzyme A Causes Detachment of Alveolar Epithelial A549 Cells Accompanied by Promotion of Interleukin-8 Release
Granzyme A Causes Detachment of Alveolar Epithelial A549 Cells Accompanied by Promotion of Interleukin-8 Release
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DOI：
10.1271/bbb.80362
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发表时间：
2008-09
期刊：
影响因子：
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通讯作者：
Yumiko Yoshikawa;Hirofumi Hirayasu;S. Tsuzuki;T. Fushiki
中科院分区：
文献类型：
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作者：
Yumiko Yoshikawa;Hirofumi Hirayasu;S. Tsuzuki;T. Fushiki
颗粒酶A（GrA）是在细胞毒性淋巴细胞、肺上皮细胞（肺泡II型细胞）和肺泡巨噬细胞中产生的丝氨酸蛋白酶。在本研究中，重组大鼠GrA（rGrA）被发现会导致肺泡II型上皮细胞系A549变圆和脱落。此外，rGrA刺激释放的中性粒细胞趋化因子，白细胞介素-8，从细胞中，通过一种机制，涉及微管破坏，可能是由于减少细胞粘附到培养皿。这些结果表明，GrA可能参与某些肺部疾病的发病机制，其特征在于肺泡壁结构的损失，中性粒细胞积聚和慢性炎症。
Granzyme A (GrA) is a serine protease produced in cytotoxic lymphocytes, lung epithelial cells (alveolar type-II cells), and alveolar macrophages. In the present study, recombinant rat GrA (rGrA) was found to cause rounding and detachment of an alveolar type-II epithelial cell line, A549. Also, rGrA stimulated release of a neutrophil chemoattractant, interleukin-8, from the cells, via a mechanism involving microtubule disruption, probably resulting from reduction of cell adhesion to culture dishes. These findings suggest that GrA might be involved in the pathogenesis of certain lung diseases characterized by loss of alveolar wall structures, neutrophil accumulation, and chronic inflammation.