Effect of different doses of dietary calcium on murine colonic cell proliferation.
Effect of different doses of dietary calcium on murine colonic cell proliferation.
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DOI：
10.1097/00008469-199403000-00007
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发表时间：
1994-03-01
期刊：
影响因子：
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通讯作者：
Martin, F
中科院分区：
文献类型：
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作者：
Piard, F;Martin, M;Martin, F
120只6周龄257 BL/65小鼠用补充有胆汁酸和钙的不同组合的饮食喂养2或7周。用多因素方差分析研究了钙、胆汁酸和这些处理的持续时间对结肠粘膜增殖指数的影响。在未用胆汁酸处理的小鼠中，与补充0.5%和1%钙的饮食相比，饮食中低水平（0.1%）的钙与隐窝每个隔室中显著更高的细胞数量相关（P < 0.01）。正常和高钙饮食组之间没有差异。无论治疗持续时间如何，胆汁酸均显著增加所有动物组的增殖指数;然而，饲喂0.5%和1%钙的小鼠的这种作用显著低于饲喂0.1%钙的小鼠（P < 0.01）。在标记细胞数和标记指数方面，胆汁酸的作用和钙的作用之间存在显著的交互作用。治疗持续时间对这些指标的影响不大。补充钙可显著降低胆汁酸对结肠增殖活性的影响，且这种影响随时间推移而稳定。虽然最高钙饮食没有毒性作用，但将钙剂量增加超过0.5%没有优势。
One-hundred and twenty 6-week-old 257BL/65 mice were fed for 2 or 7 weeks with diets supplemented with different combinations of bile acids and calcium. The effect of calcium, bile acids and the duration of these treatments on proliferative indices of the colonic mucosa was studied with a multiway analysis of variance. In mice not treated with bile acids, a low level (0.1%) of calcium in the diet was related to a significantly higher number of cells in each compartment of the crypt, compared with diets supplemented with 0.5 and 1% calcium (P < 0.01). There was no difference between the groups fed with normal and high calcium diets. Bile acids significantly increased proliferative indices in all animal groups whatever the duration of the treatment; however, this effect was significantly lower in the mice fed with 0.5% and 1% calcium than in those fed with 0.1% calcium (P < 0.01). There was a significant interaction between the effect of bile acids and the effect of calcium regarding the number of labeled cells and the labeling indices. Duration of the treatment had little effect on these indices. The effect of bile acids on colonic proliferative activity could be significantly reduced by calcium supplementation, and this effect was stable with time. Although there was no toxic effect of the highest calcium diet, there was no advantage in increasing the calcium dose beyond 0.5%.