Characterization of CYP11A1 and its potential role in sex asynchronous gonadal development of viviparous black rockfish Sebastes schlegelii (Sebastidae)
Characterization of CYP11A1 and its potential role in sex asynchronous gonadal development of viviparous black rockfish Sebastes schlegelii (Sebastidae)
复制标题
CYP11A1 的表征及其在胎生黑石斑鱼 Sebastes schlegelii（Sebastidae）性异步性腺发育中的潜在作用
DOI：
10.1016/j.ygcen.2020.113689
复制
发表时间：
2021-02-01
影响因子：
2.7
通讯作者：
Qi, Xin
中科院分区：
文献类型：
--
作者：
Wang, Xiaojie;Wen, Haishen;Qi, Xin
由 cyp11a1 基因编码的线粒体细胞色素 P450 侧链裂解 (P450scc) 启动类固醇生物合成的第一步。在本研究中，克隆了黑石斑鱼（Sebastes schlegelii）cyp11a1 的 1554 bp 开放阅读框（ORF）。 cyp11a1基因位于5号染色体上，有9个外显子。 ORF 编码 517 个氨基酸的推定前体蛋白，预测的可切割线粒体靶向肽位于氨基酸 1-39。 P450scc与其他硬骨鱼和四足动物具有同源性，它们与血红素、胆固醇和肾上腺素结合区域相对保守。组织分布分析显示，在成熟性腺和头肾中检测到cyp11a1的表达水平最高，但在妊娠/退化卵巢、退化睾丸和其他组织中检测到低水平。在睾丸 Leydig 细胞、卵巢卵泡膜细胞、头肾肾间腺、垂体和大脑多个区域中观察到 P450scc 的免疫染色。特别是，在黑石斑鱼中发现了两种鱼类特有的P450scc阳性细胞，包括脑血管冠状细胞和垂体生长激素细胞。我们的结果为 P450scc 通过介导胎生硬骨鱼类固醇生成在繁殖行为中发挥的潜在作用提供了新的证据。
Mitochondrial cytochrome P450 side-chain cleavage (P450scc), encoded by the cyp11a1 gene, initiates the first step of steroid biosynthesis. In this study, a 1554-bp open reading frame (ORF) of black rockfish (Sebastes schlegelii) cyp11a1 was cloned. The cyp11a1 gene is located on chromosome 5 and has 9 exons. The ORF encodes a putative precursor protein of 517 amino acids, and the predicted cleavable mitochondrial targeting peptide is located at amino acids 1-39. P450scc shares homology with other teleosts and tetrapods, which have relatively conserved binding regions with heme, cholesterol and adrenodoxin. Tissue distribution analysis revealed that the highest expression levels of cyp11a1 were detected in mature gonads and head kidney but that low levels were detected in gestational/regressed ovaries, regressed testes and other tissues. Immunostaining of P450scc was observed in testicular Leydig cells, ovarian theca cells, interrenal glands of head kidney, pituitary and multiple regions of brain. Particularly, two kinds of fish-specific P450scc-positive cells, including coronet cells of brain saccus vasculosus and hypophyseal somatolactin cells, were identified in black rockfish. Our results provide novel evidence for the potential role played by P450scc in reproduction behavior by mediating steroidogenesis in viviparous teleost.