Arabidopsis JAZ Proteins Interact with and Suppress RHD6 Transcription Factor to Regulate Jasmonate-Stimulated Root Hair Development
Arabidopsis JAZ Proteins Interact with and Suppress RHD6 Transcription Factor to Regulate Jasmonate-Stimulated Root Hair Development
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拟南芥JAZ蛋白与RHD6转录因子相互作用并抑制RHD6转录因子以调节茉莉酸刺激的根毛发育
DOI：
10.1105/tpc.19.00617
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发表时间：
2020-04-01
期刊：
影响因子：
11.6
通讯作者：
Hu, Yanru
中科院分区：
文献类型：
--
作者：
Han, Xiao;Zhang, Minghui;Hu, Yanru
根毛起源于毛细胞，对于植物的固着、营养获取和环境相互作用至关重要。已知植物激素茉莉酸调节拟南芥根毛发育，但关于茉莉酸在此过程中调节的分子机制知之甚少。在这里，我们表明，外源茉莉酸的应用显着刺激根毛伸长，但相反，阻断茉莉酸的感知或信号导致根毛缺陷。茉莉酸持续提高了几个关键基因的表达水平，这些基因积极参与根毛的生长。机制研究表明，茉莉酸酯ZIM-DOMAIN（JAZ）蛋白，茉莉酸信号传导的关键阻遏物，与根毛缺陷6（RHD 6）和RHD 6 LIKE 1（RSL 1），两个转录因子是根毛发育所必需的物理相互作用。JAZ蛋白抑制RHD 6的转录功能，干扰RHD 6与RSL 1的相互作用。遗传分析表明，茉莉酸促进根毛生长的RHD 6/RSL 1依赖的方式。此外，RHD 6的过表达在很大程度上挽救了积累JAZ的植物的根毛缺陷。总之，我们的研究揭示了茉莉酸途径的JAZ阻遏物直接调控RHD 6和RSL 1转录因子整合茉莉酸信号和根毛发育过程的关键信号模块，茉莉酸途径的JAZ阻遏物直接与RHD 6和RSL 1转录因子相互作用并抑制RHD 6和RSL 1转录因子整合茉莉酸信号和根毛发育过程。
Root hairs arise from trichoblasts and are crucial for plant anchorage, nutrient acquisition, and environmental interactions. The phytohormone jasmonate is known to regulate root hair development in Arabidopsis (Arabidopsis thaliana), but little is known about the molecular mechanism underlying jasmonate modulation in this process. Here, we show that the application of exogenous jasmonate significantly stimulated root hair elongation, but, on the contrary, blocking the perception or signaling of jasmonate resulted in defective root hairs. Jasmonate consistently elevated the expression levels of several crucial genes positively involved in root hair growth. Mechanistic investigation revealed that JASMONATE ZIM-DOMAIN (JAZ) proteins, critical repressors of jasmonate signaling, physically interacted with ROOT HAIR DEFECTIVE 6 (RHD6) and RHD6 LIKE1 (RSL1), two transcription factors that are essential for root hair development. JAZ proteins inhibited the transcriptional function of RHD6 and interfered with the interaction of RHD6 with RSL1. Genetic analysis indicated that jasmonate promoted root hair growth in a RHD6/RSL1-dependent manner. Moreover, overexpression of RHD6 largely rescued the root hair defects of JAZ-accumulating plants. Collectively, our study reveals a key signaling module in which JAZ repressors of the jasmonate pathway directly modulate RHD6 and RSL1 transcription factors to integrate jasmonate signaling and the root hair developmental process.JAZ repressors of the jasmonate pathway directly interact with and suppress RHD6 and RSL1 transcription factors to integrate jasmonate signaling and root hair development in Arabidopsis.