Clinical-grade Garcinia cambogia extract dissolves calcium oxalate crystals in Drosophila kidney stone models
Clinical-grade Garcinia cambogia extract dissolves calcium oxalate crystals in Drosophila kidney stone models
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DOI：
10.26355/eurrev_202006_21542
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发表时间：
2020-06-01
影响因子：
3.3
通讯作者：
Zhang, F-J
中科院分区：
文献类型：
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作者：
Fan, Q-X;Gong, S-Q;Zhang, F-J
目的：肾结石患者术后结石复发率高，且无有效药物治疗。羟基柠檬酸（HCA）。黄藤提取物的主要成分黄藤提取物能在体外溶解草酸钙晶体，提示黄藤提取物可用于治疗草酸钙肾结石。在本研究中，我们采用果蝇肾脏疾病模型来评估藤黄果对体内预防和清除草酸钙结石的作用。材料与方法：用含不同浓度GCE的蝇食饲养1周。考察了GCE对防止草酸钙结石形成的作用。WT和v-ATPase基因RNAi敲低的果蝇在含有0.3% NaOx的蝇粮中饲养一周，然后饲喂不同浓度的GCE一周。考察了GCE对草酸钙结石的去除效果。结果：与柠檬酸相比，藤黄果提取物在遗传性和非遗传性果蝇肾结石模型中均能溶解马氏小管中的草酸钙晶体。羟基柠檬酸还能直接溶解果蝇体内马氏小管中的草酸钙晶体。结论：藤黄果提取物通过HCA直接溶解草酸钙结石去除果蝇小管中的草酸钙结石。我们的研究强烈提示临床级藤黄果提取物可用于治疗肾结石患者在未来。
OBJECTIVE: Kidney stone formers have a high rate of stone recurrence after kidney stone removal surgery and there is no effective medication for treatment. Hydroxycitric acid (HCA). which is the major component of Garcinia cambogia extract, can dissolve calcium oxalate crystals in vitro, suggesting that Garcinia cambogia could be used to treat calcium oxalate kidney stone. In this study, we used the Drosophila kidney disease model to evaluate the effect of Garcinia cambogia on the prevention and removal of calcium oxalate stones in vivo.MATERIALS AND METHODS: Flies were reared in fly food containing different concentrations of GCE for one week. The effect of GCE on preventing the formation of calcium oxalate stone was examined. WT and v-ATPase gene RNAi knockdown flies were reared in fly food with 0.3% NaOx for one week, then fed different concentrations of GCE for one week. The effect of GCE on the removal of calcium oxalate stone was examined.RESULTS: Garcinia cambogia extract dissolves calcium oxalate crystals from Malpighian tubules in both genetic and non-genetic Drosophila kidney stone models compared to citric acid. Hydroxycitric acid also directly dissolves calcium oxalate crystals in Drosophila Malpighian tubules ex vivo.CONCLUSIONS: Garcinia cambogia extract removes calcium oxalate kidney stones from Drosophila Malpighian tubules via directly dissolving calcium oxalate stones by HCA. Our study strongly suggests that clinical-grade Garcinia cambogia extract could be used to treat patients with nephrolithiasis in the future.