DIFFERENTIAL-EFFECTS OF CAPSAICIN ON THE CONTENT OF SOMATOSTATIN, SUBSTANCE-P, AND NEUROTENSIN IN THE NERVOUS-SYSTEM OF THE RAT
DIFFERENTIAL-EFFECTS OF CAPSAICIN ON THE CONTENT OF SOMATOSTATIN, SUBSTANCE-P, AND NEUROTENSIN IN THE NERVOUS-SYSTEM OF THE RAT
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DOI：
10.1007/bf00500070
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发表时间：
1981-01-01
影响因子：
3.6
通讯作者：
LEMBECK, F
中科院分区：
文献类型：
--
作者：
GAMSE, R;LEEMAN, SE;LEMBECK, F
本实验观察了免疫活性物质P物质(I-SP)、生长抑素(I-SRIF)和神经降压素(I-NT)在大鼠周围神经系统和中枢神经系统的分布及辣椒素对其浓度的影响。新生儿辣椒素治疗(50 mg/kg皮下注射)导致感觉神经和脊髓后半部I-SRIF和I-SP的耗竭。检查4mo时，多肽含量未见恢复。后来暗示了一种不可逆转的效应。I-NT不是初级感觉神经元的组成部分，在脊髓内未见改变。没有一项多肽研究是在下丘脑或视前区耗尽的。辣椒素处理的成年大鼠初级感觉神经元的I-SRIF和I-SP也明显减少。使用的最高剂量(950 mg/kg S.C.)除背根神经节内的I-SP外，其余各剂量组的耗竭程度均不大于50 mg/kg剂量组。注射辣椒素导致的耗竭程度高于S.C.治疗1wk后复查给药情况。与新生儿治疗相比，I-SRIF含量在4mo内完全恢复。成年大鼠治疗后。I-SP含量在角膜、迷走神经、脊髓背侧和延髓均未完全恢复，但持续下降达9个月。侧脑室注射辣椒素(200微克)可引起延髓内I-SP的耗竭，但对下丘脑或视前区3种多肽的含量无影响。与全身治疗相比，三叉神经节内未发现I-SP或I-SRIF的耗竭。在脑室内或全身治疗后，眼睛的化疗敏感性被消除。重复局部应用辣椒素溶液(10 mg/ml)在4h内导致角膜中i-SP几乎完全耗尽。辣椒素处理引起初级感觉神经元I-SRIF和I-SP的耗竭。虽然局部或全身注射辣椒素会导致终末耗竭，但脑室注射辣椒素未能耗尽三叉神经节中的多肽，这表明整个神经元的耗竭需要辣椒素对外周分支和/或细胞体的作用。
The distribution of immunoreactive substance P (I-SP), somatostatin (I-SRIF) and neurotensin (I-NT) and the effect of capsaicin treatment on the concentration of these peptides were studied in the peripheral nervous system and CNS of the rat. Neonatal capsaicin treatment (50 mg/kg s.c.) caused a depletion of I-SRIF as well as of I-SP in sensory nerves and in the dorsal half of the spinal cord. No recovery of the peptide content was found when examined 4 mo. later suggesting an irreversible effect. I-NT, not a constituent of primary sensory neurons, was not changed in the spinal cord. None of the peptides studies was depleted in the hypothalamus or preoptic area. Capsaicin treatment of adult rats also led to a decrease of I-SRIF and I-SP in primary sensory neurons. The highest dose used (950 mg/kg s.c.) induced no greater depletion than the lowest one (50 mg/kg), except for I-SP in dorsal root ganglia. Capsaicin injection led to a higher degree of depletion than s.c. administration as examined 1 wk after treatment. In contrast to neonatal treatment, the I-SRIF content was completely restored within 4 mo. after treatment of adult rats. The I-SP content did not completely recover in all areas but remained reduced in cornea, vagus nerve, dorsal spinal cord, and medulla oblongata for up to 9 mo. Intraventricular administration of capsaicin (200 .mu.g) caused a depletion of I-SP in the medulla oblongata but had no effect on the content of all 3 peptides in hypothalamus or preoptic area. In contrast to systemic treatment, no depletion of I-SP or I-SRIF was found in the trigeminal ganglion. Chemosensitivity of the eye was abolished after intraventricular or systemic treatment. Repeated topical application of a capsaicin solution (10 mg/ml) to the eye led within 4 h to a nearly complete depletion of I-SP in the cornea. Capsaicin treatment of rats caused a depletion of both I-SRIF and I-SP in primary sensory neurons. While topical or systemic capsaicin administration caused depletion in terminals, the failure of intraventricular injections of capsaicin to deplete the peptides in the trigeminal ganglion suggests that depletion of the entire neuron requires an action of capsaicin on the peripheral branch and/or the cell body.