Atrial natriuretic factor induced phosphorylation of human placental membrane protein: an effect mimicked by guanosine 3':5'-cyclic monophosphate.
Atrial natriuretic factor induced phosphorylation of human placental membrane protein: an effect mimicked by guanosine 3':5'-cyclic monophosphate.
复制标题
心房钠尿因子诱导人胎盘膜蛋白磷酸化：鸟苷 3:5-环单磷酸模仿的效果。
DOI：
10.1016/s0006-291x(86)80009-2
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发表时间：
1986
影响因子：
3.1
通讯作者：
Roy,P
中科院分区：
文献类型：
--
作者：
Sen,I;Roy,P
心房利钠因子（ANF）特异性刺激了人胎盘分离膜片段中一个蛋白带的内源性磷酸化。sds -聚丙烯酰胺凝胶电泳测定底物蛋白的表观分子量为16 ~ 17万。在相同的膜组分中，ANF也以剂量依赖的方式刺激鸟苷酸环化酶活性。代替ANF加入到膜组分中的鸟苷3′：5′-环单磷酸鸟苷（cyclic GMP）也刺激了几个蛋白带的磷酸化，其中一个与ANF刺激的蛋白带具有相同的表观分子量。相比之下，类似浓度的腺苷3′：5′-环单磷酸腺苷（cyclic AMP）和血管紧张素II、胰岛素、加压素等激素对该蛋白带的磷酸化状态没有影响。发现ANF改变某种膜蛋白的磷酸化状态，并且这种作用被环gmp模仿，这表明至少ANF的一些生物作用可能是由涉及环gmp依赖性蛋白激酶的膜蛋白磷酸化介导的。
Atrial natriuretic factor (ANF) specifically stimulated the endogenous phosphorylation of a protein band in an isolated membrane fraction of human placenta. The apparent molecular weight of the substrate protein as determined by SDS-polyacrylamide gel electrophoresis is 160–170, 000. In the same membrane fraction, ANF also stimulated guanylate cyclase activity in a dose-dependent manner. Guanosine 3′: 5′-cyclic monophosphate (cyclic GMP), added to the membrane fraction in lieu of ANF, also stimulated the phosphorylation of several protein bands, one of which have the same apparent molecular weight as the one stimulated by ANF. In contrast, adenosine 3′: 5′-cyclic monophosphate (cyclic AMP) at a similar concentration and hormones such as angiotensin II, insulin and vasopressin had no effect on the phosphorylation state of this protein band.The finding that ANF alters the phosphorylation state of a certain membrane protein and that this effect is mimicked by cyclic-GMP suggests that at least some of the biological action of ANF may be mediated by the phosphorylation of membrane protein involving a cyclic GMP-dependent protein kinase.