Undibacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond.
Undibacterium macrobrachii sp. nov., isolated from a freshwater shrimp culture pond.
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巨臂Undibacteria sp.
DOI：
10.1099/ijs.0.057661-0
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发表时间：
2014
影响因子：
2.8
通讯作者：
Wen‐ming chen
中科院分区：
文献类型：
--
作者：
Shih;Yang;Jhen;Wen‐ming chen
从台湾淡水虾养殖池中分离到一株细菌，命名为CMJ-9（T），并使用多相分类方法对其进行了表征。菌株CMJ-9（T）为严格需氧菌，革兰氏阴性，单极鞭毛运动，含聚β-羟基丁酸酯，形成淡黄色菌落。生长发生在10 - 37 ° C（最适，20 - 30 ° C），具有0 - 0.8%NaCl（最适，0 - 0.1%）和pH 6.0 - 9.0（最适，pH 6.0 - 7.0）。 系统发育分析表明，菌株CMJ-9（T）属于Undibacterium属，与其最近的邻属是Undibacterium seohonense SHS 5 - 24（T），同源性为96.7%。 主要的细胞脂肪酸是特征3（C16：1 ω 7c和/或C16：1 ω 6c）和C16：0的总和。 细胞内羟基脂肪酸主要为C10：0 3-OH。 极性脂质谱由主要脂质磷脂酰乙醇胺、磷脂酰甘油和二磷脂酰甘油组成。多胺谱由主要化合物腐胺和中等量的2-羟基腐胺组成。呼吸醌主要为Q-8，DNA G + C含量为47.7mol%。根据系统发育和表型数据，菌株CMJ-9（T）应被归类为一个新种，建议将其命名为大臂Undibacterium macrobrachii sp. nov.。模式菌株为CMJ-9（T）（= BCRC 80406（T）= LMG 26891（T）= KCTC 23916（T））。
A bacterial strain designated CMJ-9(T) was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-9(T) were strictly aerobic, Gram-negative, motile by a single polar flagellum, poly-β-hydroxybutyrate-containing and formed light-yellow colonies. Growth occurred at 10-37 °C (optimum, 20-30 °C), with 0-0.8 % NaCl (optimum, 0-0.1 %) and at pH 6.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-9(T) belonged to the genus Undibacterium, and its closest neighbour was Undibacterium seohonense SHS5-24(T), with 96.7 % sequence similarity. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The major cellular hydroxy fatty acid was C10 : 0 3-OH. The polar lipid profile consisted of the predominant lipids phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 47.7 mol%. On the basis of the phylogenetic and phenotypic data, strain CMJ-9(T) should be classified within a novel species, for which the name Undibacterium macrobrachii sp. nov. is proposed. The type strain is CMJ-9(T) ( = BCRC 80406(T) = LMG 26891(T) = KCTC 23916(T)).
DOI：
10.1099/ijs.0.038075-0
发表时间：
2012-03-01
影响因子：
2.8
作者：
Kim, Ok-Sun;Cho, Yong-Joon;Chun, Jongsik
通讯作者：
Chun, Jongsik