LYMPHOCYTES-B EXPRESS AND LOSE SYNDECAN AT SPECIFIC STAGES OF DIFFERENTIATION
LYMPHOCYTES-B EXPRESS AND LOSE SYNDECAN AT SPECIFIC STAGES OF DIFFERENTIATION
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DOI：
10.1091/mbc.1.1.27
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发表时间：
1989-11-01
期刊：
影响因子：
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通讯作者：
BERNFIELD, M
中科院分区：
文献类型：
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作者：
SANDERSON, RD;LALOR, P;BERNFIELD, M
淋巴细胞的正常成熟需要与骨髓基质相互作用，并在分化过程中表现出与基质的粘附变化。Syndecan是一种富含硫酸肝素的整体膜蛋白多糖，通过将细胞与间质胶原、纤维连接蛋白和血栓反应蛋白结合而发挥基质受体的作用。因此，我们询问syndecan是否存在于淋巴细胞表面。在骨髓中，我们只在前体B细胞上发现syndecan。随着骨髓中b细胞的成熟和间质基质中b淋巴细胞向浆细胞的分化，表达发生变化。B细胞前体上的Syndecan比浆细胞上的更不均匀，稍大。Syndecan 1)不是在b淋巴细胞成熟和释放到循环之前立即表达，2)在循环和外周b淋巴细胞中不存在，3)在它们分化为固定浆细胞时重新表达。因此，syndecan仅在B淋巴细胞与细胞外基质相关时表达。这些结果表明，B细胞在体内的分化改变了它们的基质受体表达，并提示syndecan在B细胞阶段特异性粘附中的作用。
Lymphopoietic cells require interactions with bone marrow stroma for normal maturation and show changes in adhesion to matrix during their differentiation. Syndecan, a heparan sulfate-rich integral membrane proteoglycan, functions as a matrix receptor by binding cells to interstitial collagens, fibronectin, and thrombospondin. Therefore, we asked whether syndecan was present on the surface of lymphopoietic cells. In bone marrow, we find syndecan only on precursor B cells. Expression changes with pre-B cell maturation in the marrow and with B-lymphocyte differentiation to plasma cells in interstitial matrices. Syndecan on B cell precursors is more heterogeneous and slightly larger than on plasma cells. Syndecan 1) is not immediately before maturation and release of b lymphocytes into the circulation, 2) is absent on circulating and peripheral B lymphocytes, and 3) is reexpressed upon their differentiation into immobilized plasma cells. Thus, syndecan is expressed only when and where B lymphocytes associate with extracellular matrix. These results indicate that B cells differentiating in vivo alter their matrix receptor expression and suggest a role for syndecan in B cell stage-specific adhesion.