Depletion of Langerhans cells in the tongue from patients with advanced-stage acquired immune deficiency syndrome: relation to opportunistic infections
Depletion of Langerhans cells in the tongue from patients with advanced-stage acquired immune deficiency syndrome: relation to opportunistic infections
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DOI：
10.1111/j.1365-2559.2011.04068.x
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发表时间：
2012-02-01
期刊：
影响因子：
6.4
通讯作者：
Vargas, Pablo A.
中科院分区：
文献类型：
--
作者：
Gondak, Rogerio O.;Alves, Daniel B.;Vargas, Pablo A.
目的：利用尸检材料，对不同机会性感染的艾滋病患者舌粘膜、获得性免疫缺陷综合征（AIDS）患者舌粘膜和正常舌粘膜中郎格罕细胞（LC）的表达进行定量和比较。人类白细胞抗原D相关（HLA-DR），应用免疫组化方法检测40例艾滋病患者舌组织中LCs的表达，并对LCs进行定量分析（10例舌念珠菌病、10例舌疱疹、10例口腔毛状白斑和10例无病变）和23例人类免疫缺陷病毒（HIV）阴性对照患者的舌。通过常规形态测量法在舌的四个不同区域（前、中、后和侧）进行定量。结果表示为每面积上皮的阳性细胞。与非艾滋病对照组相比，艾滋病患者舌各部位的CD 1a（+）细胞密度（P < 0.001）、HLA-DR（P < 0.003）和CD 83（P < 0.001）均明显降低。然而，没有发现任何差异的标志物时，艾滋病患者不同的机会性感染与艾滋病患者无舌infection.Conclusions：晚期艾滋病患者表现出损耗的LC在舌粘膜。HIV感染诱导LC的细胞病变变化，无论是否存在口腔感染，都会导致LC耗竭。
Aims: To quantify and compare the expression of Langerhans cells (LCs) in the tongue mucosa of AIDS patients with different opportunistic infections, and from acquired immune deficiency syndrome (AIDS) and non-AIDS patients with normal tongues, using autopsy material.Methods and results: Human leucocyte antigen D-related (HLA-DR), CD1a and CD83 antibodies were used to identify and quantify LCs by immunohistochemistry in tongue tissue of 40 AIDS patients (10 with lingual candidiasis, 10 with lingual herpes, 10 with oral hairy leukoplakia and 10 with no lesions) and 23 tongues from human immunodeficiency virus (HIV)negative control patients. Quantification was performed by means of conventional morphometry in four different regions (anterior, middle, posterior and lateral) of the tongue. The results were expressed as positive cells per area of epithelium. The AIDS patients presented a lower density of CD1a(+) cells (P < 0.001), HLA-DR (P < 0.003) and CD83 (P < 0.001) in all regions of the tongue compared to the non-AIDS control group. However, no differences in any of the markers were found when AIDS patients with different opportunistic infections were compared with AIDS patients without tongue infection.Conclusions: Advanced stage AIDS patients showed a depletion of LCs in the tongue mucosa. HIV infection induces cytopathic changes in LCs, contributing to their depletion regardless of the presence of oral infections.