Enhanced expression of decay-accelerating factor and CD59/homologous restriction factor 20 in intestinal metaplasia, gastric adenomas and intestinal-type gastric carcinomas but not in diffuse-type carcinomas
Enhanced expression of decay-accelerating factor and CD59/homologous restriction factor 20 in intestinal metaplasia, gastric adenomas and intestinal-type gastric carcinomas but not in diffuse-type carcinomas
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DOI：
10.1046/j.1365-2559.2002.01350.x
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发表时间：
2002-04-01
期刊：
影响因子：
6.4
通讯作者：
Tsuji, T
中科院分区：
文献类型：
--
作者：
Kiso, T;Mizuno, M;Tsuji, T
目的：补体调节蛋白、衰变加速因子、CD59/同源限制因子20(CD59/HRF20)和膜辅助因子蛋白在人类胃肠道恶性肿瘤中有不同程度的表达，但其在胃癌中的表达尚未完全阐明。因此，我们用免疫组织化学方法确定了这些蛋白在人正常胃粘膜中的分布。方法：经胃镜活检或手术切除胃组织，用鼠抗衰变加速因子、CD59/HRF20和膜辅助因子蛋白的单抗进行染色。在正常胃粘膜中，膜辅助因子蛋白广泛表达于上皮细胞的基底外侧表面，而衰变加速因子和CD59/HRF20的表达不明显。在肠化生、腺瘤和肠型胃癌细胞中，衰变加速因子和HRF20在根尖表面呈强阳性表达，膜辅助因子蛋白在基底外侧表面仍有表达。在弥漫型胃癌中，衰变加速因子CD59/HRF20表达缺失，但肿瘤细胞表面存在膜辅助因子蛋白。结论：膜辅助因子蛋白在正常和肿瘤细胞补体活化的调节中起主要作用，补体调节蛋白的表达模式与胃癌的发生发展密切相关。
Aims: Variable expression of the complement regulatory proteins, decay-accelerating factor, CD59/ homologous restriction factor 20 (HRF20) and membrane cofactor protein has been shown in human gastrointestinal malignancies, but their expression in gastric cancer has not been fully described. Thus, we immunohistochemically defined the distribution of these proteins in human normal gastric mucosa. intestinal metaplasia, adenomas and gastric cancers.Methods and results: Gastric tissues were obtained by endoscopic biopsy or surgical resection and stained with mouse monoclonal antibodies to decay-accelerating factor, CD59/HRF20, and membrane cofactor protein. In the normal gastric mucosa, membrane cofactor protein was diffusely stained on the basolateral surface of epithelial cells, whereas the expression of decay-accelerating factor and CD59/HRF20 was inconspicuous. In intestinal metaplasia, adenoma and intestinal-type gastric carcinoma cells, decay-accelerating factor and HRF20 were intensely stained on the apical surface; membrane cofactor protein retained its location on the basolateral surface. In diffuse-type gastric carcinomas, the expression of decay-accelerating factor, CD59/HRF20 was lost, but membrane cofactor protein was present on the tumour cell surface.Conclusions: These findings suggest that membrane cofactor protein plays a primary role in the regulation of complement activation in normal and neoplastic gastric cells and that the expression pattern of the complement regulatory proteins is closely related to gastric carcinoma development.