Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization
Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization
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DOI：
10.1007/s11434-008-0399-x
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发表时间：
2008-09
影响因子：
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通讯作者：
C. Yi;Shuzhu Tang;Yong Zhou;G. Liang;Z. Gong;M. Gu
中科院分区：
文献类型：
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作者：
C. Yi;Shuzhu Tang;Yong Zhou;G. Liang;Z. Gong;M. Gu
选用sativaandO。latifoliate分别属于foryza的AA和CCDD基因组。本研究利用胚胎抢救技术获得了这些物种的种间杂种。杂交种的穗部性状，如芒长、粒小、外生的紫色大柱头、籽粒碎裂和分散的穗部等，与父本O。而在株高、分蘖能力和营养活力等方面存在明显的杂种优势。随后，利用基因组素原位杂交（GISH）和荧光素原位杂交（FISH）分析研究了杂交后代的染色体配对和基因组成分。根据对根尖有丝分裂中期染色体数目的研究，该杂种为三倍体，有36条染色体。该杂交种的基因组结构为ACD。在花粉母细胞减数分裂中期，杂种花粉母细胞的染色体配对较差，大部分染色体为单价，导致杂种花粉完全雄性不育。
Oryza sativaandO. latifoliabelong to the AA and CCDD genomes ofOryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent,O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomicin situhybridization (GISH) and fluorescentin situhybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chromosome pairing was identified and most of the chromosomes were univalent, which resulted in complete male sterility in the hybrid.