Long term exposure to retinoic acid induces the expression of IRK1 channels in HERG channel-endowed neuroblastoma cells
Long term exposure to retinoic acid induces the expression of IRK1 channels in HERG channel-endowed neuroblastoma cells
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DOI：
10.1006/bbrc.1998.8319
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发表时间：
1998-03-27
影响因子：
3.1
通讯作者：
Olivotto, M
中科院分区：
文献类型：
--
作者：
Arcangeli, A;Rosati, B;Olivotto, M
研究了人神经母细胞瘤SH-SY 5 Y细胞向神经元分化过程中内向K+电导的调节。在标准培养条件下，这些细胞表达herg基因，HERG电流是调节其V-静息的主要内向K+电流。在暴露于维甲酸（RA）10-20天后，SH-SY 5 Y细胞除了HERG：电流外，还显示出一种新的电流，其特征在于向内整流、依赖于细胞外K+浓度以及被Cs+和Ba 2+阻断，这是IRK 1电流的主要特征。该电流的出现伴随着V-静止的强超极化。RT-PCR实验证实，IRK 1（Kir 2.1）基因的转录本实际上出现在用RA处理10-20天的SH-SY 5 Y细胞中。总的来说，这里提出的数据表明，RA诱导的神经母细胞瘤细胞的神经元分化伴随着从HERG驱动的V-休息的IRK 1驱动的控制开关，类似于正常分化的神经元中发生的情况;然而，在肿瘤细胞中，这种开关并不意味着取消HERG通道表达。(C)北京：科学出版社.
The modulation of inward K+ conductances was studied during neuronal differentiation of human SH-SY5Y neuroblastoma cells. Under standard culture conditions, these cells express the herg gene, and the HERG current is the main inward K+ current regulating their V-rest. After 10-20 days exposure to Retinoic Acid (RA), SH-SY5Y cells showed, in addition to HERG: currents, a novel current characterized by inward rectification, dependence on the extracellular K+ concentration, and blockade by Cs+ and Ba2+, the main features of the IRK1 current. The appearance of this current is accompanied by a strong hyperpolarisation of V-rest. RT-PCR experiments confirmed that a transcript of the IRK1 (Kir 2.1) gene actually appears in SH-SY5Y cells treated for 10-20 days with RA. On the whole, data here presented demonstrate that RA-induced neuronal differentiation of neuroblastoma cells is accompanied by the switch from a HERG-driven to a IRK1-driven control of V-rest, similarly to what happens in normal differentiating neurons; however, in tumor cells, this switch does not imply the abolition of HERG channel expression. (C) 1998 Academic Press.