ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
ATP modulation of mitogen activated protein kinases and intracellular Ca2+ in breast cancer (MCF-7) cells
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DOI：
10.1016/j.abb.2007.07.012
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发表时间：
2007-10-01
影响因子：
3.9
通讯作者：
Santillan, Graciela
中科院分区：
文献类型：
--
作者：
Bilbao, Paola Scodelaro;Boland, Ricardo;Santillan, Graciela
In the breast tumor cell line MCF-7, extracellular nucleotides induce transient elevations in intracellular calcium concentration ([Ca2+](i)). In this study we show that stimulation with ATP or UTP sensitizes MCF-7 cells to mechanical stress leading to an additional 14 transient Ca2+ influx. ATP >= ATP7-S >= UTP >>> ADP = ADP beta-S elevate [Ca2+](i), proving the presence of P2Y(2)/P2Y(4) purinergic receptor subtypes. In addition, cell stimulation with ATP, ATP gamma-S or UTP but not ADP beta-S induced the phosphorylation of ERKI/ 2, p38 and JNK1/2 mitogen activated protein kinases (MAPKs). The use of Gd3+, La3+ or a Ca2+-free medium, inhibited ATP-dependent stress activated Ca2+ (SAC) influx, but had no effect on MAPK phosphorylation. ATP-induced activation of MAPKs was diminished by two PI-PLC inhibitors and an IP3 receptor antagonist. These results evidence an ATP-sensitive SAC influx in MCF-7 cells and indicate that phosphorylation of MAPKs by ATP is dependent on PI-PLC/IP3/C2+ i release but independent of SAC influx in these cells, differently to other cell types. (c) 2007 Elsevier Inc. All rights reserved.
