EVALUATION OF PROTEIN-QUALITY IN FISH MEALS BY CHEMICAL AND BIOLOGICAL ASSAYS
EVALUATION OF PROTEIN-QUALITY IN FISH MEALS BY CHEMICAL AND BIOLOGICAL ASSAYS
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DOI：
10.1016/0044-8486(93)90145-o
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发表时间：
1993-09-01
期刊：
影响因子：
4.5
通讯作者：
MCNIVEN, MA
中科院分区：
文献类型：
--
作者：
ANDERSON, JS;LALL, SP;MCNIVEN, MA
通过体外测定和大西洋鲑鱼（萨尔莫salar）鱼种的生长研究，评价了在加拿大大西洋地区市售的鱼粉（鲱鱼、鲱鱼和鲱鱼）和挪威鱼粉（Norse-LT 94（R））的蛋白质质量。胃蛋白酶消化率和多酶消化率可用于蛋白质品质的预测。总挥发性碱性氮，可用的赖氨酸，巯基和二硫键，特别是当单独使用时，测定的价值有限。大西洋鲑鱼鱼种（初始重量7.6 ± 0.14 g）饲喂含有两种蒸汽干燥的鲱鱼粉和Norse-LT 94（R）的饮食70天，基于重量增加、PER、NPR、NPU和斜率测定进行评价。喂食Norse-LT 94（R）的鱼比喂食加拿大餐的鱼体重增加了11-60%。加拿大鱼粉的蛋白质质量低于Norse-LT 94（R）。胃蛋白酶消化率对鱼粉的排序与生物学试验相同，但建议将确定蛋白质质量的饲料生物学试验作为最终比较方法。生物学评价是衡量饲料总体质量的首选方法，因为体外试验通常会对某些饲料的质量进行错误评级。
Fish meals (herring, menhaden, and anchovy), commercially available in Atlantic Canada and a Norwegian fish meal (Norse-LT94(R)) were evaluated for their protein quality by in vitro assays and by growth studies with Atlantic salmon (Salmo salar) fingerlings. Pepsin digestibility and multienzyme digestibility were useful in predicting protein quality. Assays for total volatile basic-nitrogen, available lysine, sulphydryl groups, and disulphide bonds, particularly when used alone, were of limited value. Atlantic salmon fingerlings (initial weight 7.6 5 +/- 0.14 g) fed diets containing two steam-dried herring meals and Norse-LT94(R) for 70 days were evaluated on the basis of weight gain, PER, NPR, NPU, and slope assay. Fish fed Norse-LT94(R) gained 11-60% more weight than fish fed the Canadian meals. The protein quality of the Canadian fish meals was lower than that of Norse-LT94(R). Pepsin digestibility ranked the fish meals in the same order as the biological tests, but the biological testing of feedstuffs to determine protein quality is recommended as the final method of comparison. Biological evaluation is the preferred method of measuring the overall quality of a feedstuff, since in vitro tests usually miss-rank the quality of some feedstuffs.