Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin
Molecular cloning and analysis of anthocyanin biosynthesis genes preferentially expressed in apple skin
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DOI：
10.1016/s0168-9452(03)00201-2
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发表时间：
2003-08-01
期刊：
影响因子：
5.2
通讯作者：
Kim, SR
中科院分区：
文献类型：
--
作者：
Kim, SH;Lee, JR;Kim, SR
花色素苷是植物的主要颜色色素。为了解苹果花色素苷的生物合成机制，从苹果（Malus arbitica Borkh.）果皮组织cDNA文库中分离了黄烷酮3-羟化酶（F3 H）、二氢黄酮醇还原酶（DFR）、花色素苷合成酶（ANS）和UDP葡萄糖：类黄酮3-O-葡萄糖基转移酶（UFGT）的cDNA。CV. Fuji）。推导出的氨基酸序列与其它植物的氨基酸序列有很高的同源性。每个基因都是一个多基因家族的成员。花色素苷生物合成基因的mRNA在皮肤组织中优先表达，并且这些基因的表达受光的协同诱导。这些基因在红皮品种的果皮中大量表达，而在非红皮品种的果皮中很少表达，表明这些基因在决定苹果果皮颜色中起重要作用。(C)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
Anthocyanin is the major color pigment in plants. In order to understand anthocyanin biosynthesis mechanism in apple, the cDNAs encoding flavanone 3-hydroxylase (F3H), dihydroflavonol reductase (DFR), anthocyanidin synthase (ANS), and UDPglucose: flavonoid 3-O-glucosyltransferase (UFGT) were isolated from cDNA libraries prepared from the skin tissues of apple (Malus domestica Borkh. cv. Fuji). Deduced amino acid sequences of the cDNAs showed high homology to the sequences from other plants. Each gene is a member of a multigene family. The mRNAs of anthocyanin biosynthetic genes were detected preferentially in the skin tissue and expression of the genes was coordinately induced by light. The transcripts were detected abundantly in the skins of cultivars with red skin, but rarely in that of a cultivar carrying non-red fruit, suggesting that these genes have major roles in determination of apple skin color. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.