PROTEIN-DEGRADATION IN 3T3-CELLS AND TUMORIGENIC TRANSFORMED 3T3-CELLS
PROTEIN-DEGRADATION IN 3T3-CELLS AND TUMORIGENIC TRANSFORMED 3T3-CELLS
复制标题
DOI：
10.1002/jcp.1041190120
复制
发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
PARDEE, AB
中科院分区：
文献类型：
--
作者：
GRONOSTAJSKI, RM;PARDEE, AB
为了研究蛋白质降解总体速率在控制细胞生长中的关系，确定成纤维细胞向致瘤性的转化是否影响其短寿命和长寿命蛋白质的降解速率。在非致瘤性小鼠 Balb/c 3T3 成纤维细胞和用不同试剂转化的致瘤性 3T3 细胞中测量蛋白质降解率。生长中的 3T3 细胞以及用莫洛尼肉瘤病毒 (MA-3T3) 或劳斯肉瘤病毒 (RS-3T3) 转化的细胞，以相似的速率降解短寿命和长寿命蛋白质。 SV40 (SV-3T3) 和苯并(a)芘 (BP-3T3) 转化细胞的短寿命和长寿命蛋白质的降解率均略低。将培养基中的血清浓度降低10%至0.5%，立即导致3T3细胞中长寿蛋白的降解率增加约2倍。转化株系在血清剥夺后仅以不同的量增加了长寿蛋白的降解率，但没有一个达到与3T3相同的程度。与3T3细胞和一些转化细胞相比，转化细胞之间的蛋白质降解率差异更大。因此，由于转化为致瘤性，3T3 细胞中蛋白质降解率没有一致的变化。
To study the relation of overall rates of protein degradation in the control of cell growth, if transformation of fibroblasts to tumorigenicity affected their rates of degradation of short- and long-lived proteins was determined. Rates of protein degradation were measured in nontumorigenic mouse Balb/c 3T3 fibroblasts, and in tumorigenic 3T3 cells transformed by different agents. Growing 3T3 cells, and cells transformed with Moloney sarcoma virus (MA-3T3) or Rous sarcoma virus (RS-3T3), degraded short- and long-lived proteins at similar rates. SV40 (SV-3T3)- and benzo(a)pyrene (BP-3T3)-transformed cells had slightly lower rates of degradation of both short- and long-lived proteins. Reducing the serum concentration in the culture medium form 10% to 0.5%, immediately caused about a 2-fold increase in the rate of degradation of long-lived proteins in 3T3 cells. Transformed lines increased their rates of degradation of long-lived proteins only by different amounts upon serum deprivation, but none of them to the same extent as did 3T3. Greater differences in the degradation rates of proteins were seen among the transformed cells than between 3T3 cells and some transformed cells. Thus, there was no consistent change in any rate of protein degradation in 3T3 cells due to transformation to tumorigenicity.