Preferential oxidation of fatty acids by rat small intestine
Preferential oxidation of fatty acids by rat small intestine
复制标题
大鼠小肠优先氧化脂肪酸
DOI：
10.1016/0014-5793(71)80557-4
复制
发表时间：
1971
期刊：
影响因子：
3.5
通讯作者：
W. C. Hülsmann
中科院分区：
文献类型：
--
作者：
W. C. Hülsmann
在该实验室的先前研究中，显示可以从大鼠小肠上皮细胞中收获线粒体，并且这些线粒体显示出完整的氧化磷酸化[11]。进一步的研究[2]表明，分离的线粒体能够将辛酸和棕榈酸完全氧化为CO？和HzO，而早期的研究[3]表明3-羟基丁酸也是这些线粒体的底物。本论文的目的是通过观察离体大鼠小肠来扩展这些研究，在体外用葡萄糖和/或长链脂肪酸、辛酸盐或3-羟基丁酸盐灌注。本实验用从进食的雄性白色大鼠（250-300 g）分离的小肠进行。动物在戊巴比妥麻醉下进行手术。操作原则上如Gerber [4]所述进行。在两条结扎线之间切开直肠并游离，然后进行完整的盲肠和大肠切除术。在离胃约5 cm处打开十二指肠，插入套管，以便用50 ml温盐水冲洗小肠。空气通过后，结扎十二指肠和末端回肠。制备不含A的腹主动脉。腹腔直到髂分叉。在主动脉周围、A的远端和近端进行松散结扎。肠系膜上级，而A.右肾被绑住了在结扎胃静脉和脾静脉后，还在门静脉和肠系膜上上级下放置了松散的结扎线。则
In a previous study from this laboratory it was shown that mitochondria can be harvested from rat small intestinal epithelial cells and that these mitochondria show intact oxidative phosphorylation [11. Further studies [2] showed that the isolated mitochondria are able to oxidize octanoate and palmitate completely to CO? and HzO, whereas earlier studies [3] showed that 3-hydroxybuty rate is also a substrate for these mitochondria. It is the purpose of the present paper to extend these studies with observations carried out with isolated rat small intestine, perfused in vitro with glucose and/or long-chain fatty acids, octanoate or 3-hydroxybutyrate.The present experiments are carried out with small intestines isolated from fed male white rats (250-300 g). The animals were operated under Nembutal narcosis. The operation was carried out in principle as described by Gerber [4]. The rectum was cut between two ligatures and mobilized, followed by a complete coecum and large bowel resection. The duodenum was opened about 5 cm from the stomach and a cannula inserted so that the small intestine could be rinsed with 50 ml warm saline. After the passage of air the duodenum and the terminal ileum were tied. The abdominal aorta was prepared free from the A. coeliaca until the bifurcatio iliaca. Loose ligatures were placed around the aorta, distal and proximal from the A. mesenterica superior, whereas the A. renalis dextra was tied. Loose ligatures were also placed under the V. porta and the V. mesenterica superior, after tying the V. gastricuduodenalis and V. lienalis. Then the