Bacterial biosurfactant in enhancing solubility and metabolism of petroleum hydrocarbons
Bacterial biosurfactant in enhancing solubility and metabolism of petroleum hydrocarbons
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DOI：
10.1016/j.jhazmat.2009.04.136
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发表时间：
2009-10-15
影响因子：
13.6
通讯作者：
Konwar, B. K.
中科院分区：
文献类型：
--
作者：
Bordoloi, N. K.;Konwar, B. K.
生物表面活性剂可以使烃类络合物具有更大的移动的性，在石油开采、原油泵送和原油污染物的生物修复中具有潜在的应用。在调查中，使用能够利用多环芳烃如菲、芘和芴的细菌分离物。观察到培养基中补充的碳氢化合物逐渐减少，细菌生物量和蛋白质相应增加。具有氟和菲的组合应用的介质引起更好的生物表面活性剂生产（0.45克升（-1））和（0.38克升（-1））由铜绿假单胞菌菌株MTCC 7815和MTCC 7814。MTCC 7815（41.0 μ g ml（-1））和MTCC 7812（26 μ g ml（-1））的生物表面活性剂对芘表现出更高的增溶作用，而MTCC 8165对菲表现出更高的增溶作用，MTCC 7812（24.45 μ g ml（-1））和MTCC 8163（24.49 μ g ml（-1））对芴表现出更高的增溶作用。生物表面活性剂MTCC 7815和MTCC 7812对芘和芴的增溶作用分别增强了它们的代谢，从而导致持续生长。生物表面活性剂的性质是脂肽和蛋白质-淀粉-脂类复合物，它们能将纯水的表面张力（72 mNm（-1））降低到35 mNm（-1）。临界胶束浓度（CMC）也低于化学表面活性剂十二烷基硫酸钠（SDS）。它们在数量和结构上有所不同。存在于生物表面活性剂中的主要鼠李糖脂是Rha-C-8-C-10和Rha-C-10-C-8。(C)2009爱思唯尔有限公司版权所有。
Biosurfactant can make hydrocarbon complexes more mobile with the potential use in oil recovery, pumping of crude oil and in bioremediation of crude oil contaminant. In the investigation, bacterial isolates capable of utilizing poly-cyclic aromatic hydrocarbons like phenanthrene, pyrene and fluorene were used. A gradual decrease of the supplemented hydrocarbons in the culture medium was observed with corresponding increase in bacterial biomass and protein. The medium having the combined application of fluorine and phenanthrene caused better biosurfactant production (0.45 g l(-1)) and (0.38 g l(-1)) by Pseudomonas aeruginosa strains MTCC7815 and MTCC7814. The biosurfactant from MTCC7815 (41.0 mu g ml(-1)) and MTCC7812 (26 mu g ml(-1)) exhibited higher solubilization of pyrene; whereas, MTCC8165 caused higher solubilization of phenanthrene; and that of MTCC7812 (24.45 mu g ml(-1)) and MTCC8163 (24.49 mu g ml(-1)) caused more solubilzation of fluorene. Higher solubilization of pyrene and fluorene by the biosurfactant of MTCC7815 and MTCC7812, respectively enhanced their metabolism causing sustained growth. Biosurfactants were found to be lipopeptide and protein-starch-lipid complex in nature and they could reduce the surface tension of pure water (72 mN m(-1)) to 35 mN m(-1). The critical micelle concentration (CMC) was also lower than the chemical surfactant sodium dodecyl sulphate (SDS). They differed in quantity and structure. The predominant rhamnolipids present in biosurfactants were Rha-C-8-C-10 and Rha-C-10-C-8. (C) 2009 Elsevier B.V. All rights reserved.