A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control
A High-Throughput Screening Method to Identify Potential Pesticides for Mosquito Control
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DOI：
10.1603/033.046.0219
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发表时间：
2009-03-01
影响因子：
2.1
通讯作者：
Linthicum, Kenneth J.
中科院分区：
文献类型：
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作者：
Pridgeon, Julia W.;Becnel, James J.;Linthicum, Kenneth J.
传播人类疾病的蚊子对国际公共卫生界至关重要。农药仍然是控制这些医学上重要物种的综合计划的主要组成部分。然而，目前登记用于控制蚊子的杀虫剂种类很少。我们的实验室创建并评估了一种使用埃及伊蚊一龄幼虫的高通量筛选方法，以快速筛选大量化学物质的抗蚊活性。使用这种高通量筛选方法测定一组代表性化合物的LC(50)值，并与通过局部施用针对雌性伊蚊成虫测定的LD(50)值进行比较。埃及伊蚊。我们的结果表明，这种高通量筛选方法适合快速筛选大量候选化学物质以鉴定有效化合物。
Mosquitoes that transmit human diseases are of major importance to the international public health community. Pesticides remain a major component of integrated programs to control these medically important species. However, very few types of pesticides are currently registered for mosquito control. A high-throughput screening method using first-instar larvae of Aedes aegypti was created and evaluated in our laboratory to quickly screen large numbers of chemicals for activity against mosquitoes. LC(50) values of a representative group of compounds were determined using this high-throughput screening method and compared with LD(50) values determined by topical application against female adults of Ae. aegypti. Our results show that this high-throughput screening method is suitable for screening large numbers of candidate chemicals quickly to identify effective compounds.