Role of nitric oxide in the local regulation of pulmonary vascular resistance in humans
Role of nitric oxide in the local regulation of pulmonary vascular resistance in humans
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DOI：
10.1161/01.cir.93.2.266
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发表时间：
1996-01-15
期刊：
影响因子：
37.8
通讯作者：
Selwyn, AP
中科院分区：
文献类型：
--
作者：
Cooper, CJ;Landzberg, MJ;Selwyn, AP
背景内皮源性一氧化氮（NO）可能是肺循环血管阻力的重要介质。我们测试了以下假设：在有意识的成年人中，内皮细胞通过NO的产生在维持基础肺血管阻力方面是重要的，并且它可以响应于受体介导的刺激而进一步增加NO的产生，方法和结果对8例37 ~ 76岁清醒成人肺动脉下叶段分布的阻力血管功能进行了研究30岁，正在接受心脏导管插入术。使用多普勒尖端导丝和定量血管造影确定节段性血流。通过输注导管在节段动脉内局部给药。N-G-单甲基-L-精氨酸（L-NMMA）被用作NO产生的特异性抑制剂，而乙酰胆碱（ACh）被用于测试受体介导的血管舒张。为了证明血管舒张ACh是NO依赖性的，ACh反应单独测试，在L-NMMA的存在下，并在控制收缩剂苯丙氨酸的存在下。基础肺血管阻力是NO依赖性的，因为L-NMMA输注导致局部血流速度呈剂量依赖性降低（P
Background Endothelium-derived nitric oxide (NO) may be an important mediator of vascular resistance in the pulmonary circulation. We tested the hypotheses that in conscious adults the endothelium, through NO production, is important in maintaining basal pulmonary vascular resistance and that it can increase NO production further in response to receptor-mediated stimulation, leading to further vasodilation.Methods and Results Pulmonary arterial resistance vessel function was studied within the distribution of a segmental lower lobe pulmonary artery in eight conscious adults 37 to 76 years old who were undergoing cardiac catheterization. Segmental blood flow was determined with use of a Doppler-tip guide wire and quantitative angiography. Drugs were administered locally within the segmental artery through an infusion catheter. N-G-Monomethyl-L-arginine (L-NMMA) was used as a specific inhibitor of NO production, whereas acetylcholine (ACh) was used to test receptor-mediated vasodilation. To demonstrate that vasodilation to ACh was NO dependent, ACh response was tested alone, in the presence of L-NMMA, and in the presence of a control constrictor phenylephrine. Basal pulmonary vascular resistance was NO dependent because L-NMMA infusion resulted in a dose-dependent decrease in local how velocity (P