Periodic and Dynamic 3-D Gold Nanoparticle-DNA Network Structures for Surface-Enhanced Raman Spectroscopy-Based Quantification
Periodic and Dynamic 3-D Gold Nanoparticle-DNA Network Structures for Surface-Enhanced Raman Spectroscopy-Based Quantification
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DOI：
10.1021/jp809949v
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发表时间：
2009-04-16
影响因子：
3.7
通讯作者：
Irudayaraj, Joseph
中科院分区：
文献类型：
--
作者：
Lee, Kyuwan;Irudayaraj, Joseph
在三维（3-D）网络结构中由DNA连接的金纳米颗粒的增强因子被评估为1.12 × 10（7），并且显示出比二维（2-D）阵列大近似10倍，这可能是由于共振的尺寸扩展和如此形成的结构的周期性。由于共振条件和激发波长（785 nm）的匹配使得能够通过表面增强拉曼光谱（Sers）对分析物进行动态定量，因此从这些DNA连接的金纳米颗粒网络中可以获得均匀和更高水平的增强。该结构首先通过获得4-巯基吡啶的Sers测量进行验证，然后，罗丹明B被用作测试分析物，以定量的染料，以纳摩尔浓度使用建议的3-D网络结构动态在液体系统中。由于这些结构可以很容易地制造，我们设想其应用在体外定量和细胞内研究在不久的将来。
The enhancement factor of gold nanoparticles linked by DNA in a three-dimensional (3-D) network structure was evaluated as 1.12 x 10(7) and shown to be greater than a two-dimensional (2-D) array by a factor of similar to 10, possibly due to the dimensional expansion of resonance and periodicity of the so formed structures. Uniform and higher level of enhancement was possible from these DNA linked gold nanoparticle networks because of the matching of the resonant condition and the excitation wavelength (785 nm) to enable dynamic quantification of analytes by surface-enhanced Raman spectroscopy (SERS). The structure was first validated by obtaining a SERS measurement of 4-mercaptopyridine; then, rhodamine B was used as a test analyte to quantify the dye up to nanomolar concentrations using the proposed 3-D network structures dynamically in a liquid system. Since these constructs can be easily fabricated, we envision its application in in vitro quantification and intracellular studies in the near future.