Construction of a high-density SSR marker-based linkage map of zoysiagrass (Zoysia japonica Steud.)
Construction of a high-density SSR marker-based linkage map of zoysiagrass (Zoysia japonica Steud.)
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DOI：
10.1007/s10681-009-9990-8
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发表时间：
2009-12-01
期刊：
影响因子：
1.9
通讯作者：
Cai, Hongwei
中科院分区：
文献类型：
--
作者：
Li, Manli;Yuyama, Nana;Cai, Hongwei
使用来自“Muroran 2”x“Tawarayama Kita 1”杂交的 86 个 F-1 个体，为结缕草（Zoysia japonica Steud.）构建了具有 447 个 SSR 标记的共有遗传连锁图谱。共有图谱鉴定出22个连锁群，总长度为2,009.9 cM，平均图谱密度为4.8 cM。与之前的 AFLP-SSR 连锁图谱相比，每个连锁组的 SSR 标记都映射到两个图谱中的相似位置。 AFLP-SSR 图谱中的八对连锁群被连接成当前图谱中的八个新群。该结缕草共有图谱包含 35 个 SSR 标记，与已知染色体位置的水稻基因组序列表现出高度同源性。这使得结缕草连锁群 2、3、9、19 和水稻染色体 3、12、2、7 之间的同线性得以分别鉴定。这些结果提供了重要的比较基因组学信息，新图谱现已可用于结缕草重要性状的数量性状基因座分析、标记辅助选择和育种。
A consensus genetic linkage map with 447 SSR markers was constructed for zoysiagrass (Zoysia japonica Steud.), using 86 F-1 individuals from the cross 'Muroran 2' x 'Tawarayama Kita 1'. The consensus map identified 22 linkage groups and had a total length of 2,009.9 cM, with an average map density of 4.8 cM. When compared with a previous AFLP-SSR linkage map, the SSR markers from each linkage group mapped to similar positions in both maps. Eight pairs of linkage groups from the AFLP-SSR map were joined into eight new groups in the current map. This zoysiagrass consensus map contained 35 SSR markers exhibiting high homology with rice genomic sequences from known chromosomal locations. This allowed synteny to be identified between Zoysiagrass linkage groups 2, 3, 9, 19 and rice chromosomes 3, 12, 2, 7 respectively. These results provide important comparative genomics information and the new map is now available for quantitative trait locus analysis, marker-assisted selection and breeding for important traits in zoysiagrass.