Comparative proteomic analysis of atrial appendages from rheumatic heart disease patients with sinus rhythm and atrial fibrillation
Comparative proteomic analysis of atrial appendages from rheumatic heart disease patients with sinus rhythm and atrial fibrillation
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DOI：
10.3892/mmr.2011.468
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发表时间：
2011-07-01
影响因子：
3.4
通讯作者：
Yang, Jin-Liang
中科院分区：
文献类型：
--
作者：
Huang, Wen-Jun;Zhou, Rui;Yang, Jin-Liang
心房颤动（AF）是临床上最常见的心律失常形式，并导致心血管疾病的发病率和死亡率。尽管对房颤相关机制的理解取得了重大进展，但有效的生物标志物和可行的治疗靶点的数量仍然相对有限。在本研究中，采用2-DE和MS/MS分析鉴定心房附件组织中AF患者（n=4）与对照组窦性心律（SR; n=5）的差异表达蛋白。所有受试者均患有风湿性心脏病。通过2-DE分析、考马斯亮蓝染色和MS/MS鉴定，发现AF组和SR组之间共有19个蛋白点存在差异表达。通过聚类和代谢/信号通路分析，将这些蛋白分为三大类：参与细胞骨架和肌丝的蛋白、能量代谢相关蛋白和氧化应激相关蛋白。本研究鉴定的蛋白可能有助于更好地了解房颤的分子机制，并可能为药物开发提供有用的生物标志物和新靶点。
Atrial fibrillation (AF) is the most common form of arrhythmia encountered in clinical practice, and contributes to cardiovascular morbidity and mortality. Despite significant advances in the understanding of the mechanisms associated with AF, the number of effective biomarkers and viable therapeutic targets remains relatively limited. In this study, 2-DE and MS/MS analysis was used to identify differentially expressed proteins in human atrial appendage tissues from patients with AF (n=4) compared to controls with sinus rhythm (SR; n=5). All subjects had rheumatic heart disease. Following 2-DE analysis, Coomassie Brilliant Blue staining and MS/MS identification, a total of 19 protein spots were found to be differentially expressed between the AF and SR groups. By cluster and metabolic/signaling pathway analysis, these proteins were divided into three major groups: proteins involved in the cytoskeleton and myofilament, energy metabolism associated proteins, and proteins associated with oxidative stress. The proteins identified in this study may enable a better understanding of the molecular mechanisms of AF, and may provide useful biomarkers and novel targets for drug development.