A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate
A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate
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DOI：
10.1104/pp.114.3.1085
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发表时间：
1997-07-01
期刊：
影响因子：
7.4
通讯作者：
Ryan, CA
中科院分区：
文献类型：
--
作者：
Heitz, T;Bergey, DR;Ryan, CA
我们研究了脂氧合酶（LOX）基因的表达与番茄（Lycopersicon esculentum）叶片中系统素依赖的创伤应答之间的关系。采用基于聚合酶链反应的方法分离出两个番茄Lox cDNA，分别称为TomLoxC和TomLoxD。TomLOXC和TomLOXD的氨基酸序列都具有约60个残基的N端延伸，体外摄取实验表明其作为转运肽发挥作用，将这些蛋白质靶向运输到叶绿体中。在创伤、系统素或茉莉酸甲酯处理后的30到50分钟内，TomLoxD的mRNA水平升高，并在1到2小时之间达到最大值。在叶片中未检测到TomLoxC的mRNA，创伤后也未发现，但是在成熟果实中发现了它，这表明这两个番茄Lox基因在不同组织中受不同过程的调控。结果表明，TomLoxD基因在叶片中因创伤而上调，并编码一种叶绿体LOX，它可能作为十八碳烯酸防御信号通路的一个组成部分发挥作用。
We investigated the relationship between the expression of lipoxygenase (LOX) genes and the systemin-dependent wound response in tomato (Lycopersicon esculentum) leaves. A polymerase chain reaction-based approach was used to isolate two tomato Lox cDNAs, called TomLoxC and TomLoxD. Both TomLOXC and TomLOXD amino acid sequences possess an N-terminal extension of about 60 residues that were shown by in vitro uptake to function as transit peptides, targeting these proteins into the chloroplast. Within 30 to 50 min following wounding or systemin or methyl jasmonate treatments, the TomLoxD mRNA level increased and reached a maximum between 1 and 2 h. TomLoxC mRNA was not detectable in leaves and was not round following wounding, but it was found in ripening fruits, indicating that the two tomato Lox genes are regulated in different tissues by different processes. The results suggest that the TomLoxD gene is up-regulated in leaves in response to wounding and encodes a chloroplast LOX that may play a role as a component of the octadecanoid defense-signaling pathway.