Neuronal calcium sensor-1 gene ncs-1a is essential for semicircular canal formation in zebrafish inner ear
Neuronal calcium sensor-1 gene ncs-1a is essential for semicircular canal formation in zebrafish inner ear
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DOI：
10.1002/neu.20138
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发表时间：
2005-09-05
期刊：
影响因子：
--
通讯作者：
Levenson, R
中科院分区：
文献类型：
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作者：
Blasiole, B;Kabbani, N;Levenson, R
我们分析了神经元钙传感器1（内斯1）在斑马鱼发育中的功能作用。我们确定了哺乳动物NCS-1基因的两个直系同源物。克隆了编码斑马鱼Ncs-1a和Ncs-1b多肽的全长cDNA并进行了表征。整体原位杂交显示，ncs-1a mRNA的表达开始于早期的体节发生。随着发育的进展，ncs-1a mRNA在整个胚胎中存在，在腹侧造血中胚层、原肾小管、CNS核和耳泡中检测到表达。在受精后4.5天（dpf），ncs-1a主要在大脑中表达。ncs-1b mRNA的表达在受精后36小时（hpf）首次检测到，并仅限于嗅球。到4.5 dpf时，ncs-1b在整个大脑中以低水平表达。用反义morpholinos敲低ncs-1a mRNA翻译阻断半规管的形成。这些研究确定了ncs-1a在内耳发育中的新功能，并表明这种钙传感器在前庭功能中起着重要作用。(c)2005 Wiley Periodicals，Inc.
We have analyzed the functional role of neuronal calcium sensor-1 (Nes-1) in zebrafish development. We identified two orthologs of the mammalian NCS-1 gene. Full-length cDNAs encoding zebrafish Ncs-1a and Ncs-1b polypeptides were cloned and characterized. Whole-mount in situ hybridization revealed that ncs-1a mRNA was expressed beginning at early somito-genesis. As development progressed, ncs-1a mRNA was present throughout the embryo with expression detected in ventral hematopoietic mesoderm, pronephric tubules, CNS nuclei, and otic vesicle. By 4.5 days post fertilization (dpf), ncs-1a expression was detected primarily in the brain. Expression of ncs-1b mRNA was first detected at 36 hours post fertilization (hpf) and was restricted to the olfactory bulb. By 4.5 dpf, ncs-1b was expressed at low levels throughout the brain. Knockdown of ncs-1a mRNA translation with antisense morpholinos blocked formation of semicircular canals. These studies identify a novel function for ncs-1a in inner ear development and suggest that this calcium sensor plays an important role in vestibular function. (c) 2005 Wiley Periodicals, Inc.