Involvement of the vacuoles of the endodermis in the early process of shoot gravitropism in Arabidopsis
Involvement of the vacuoles of the endodermis in the early process of shoot gravitropism in Arabidopsis
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DOI：
10.1105/tpc.010216
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发表时间：
2002-01-01
期刊：
影响因子：
11.6
通讯作者：
Tasaka, M
中科院分区：
文献类型：
--
作者：
Morita, MT;Kato, T;Tasaka, M
拟南芥茎的内胚层细胞被认为是感受重力的细胞。内皮层中沿重力方向沉积的造粉体可能起平衡石的作用。使用SCR启动子的SGR2和ZIG的内胚层特异性表达可以分别补充sgr 2和zig突变体的异常芽向重力性。在两个突变体中观察到的淀粉体沉降异常同时恢复，这些结果表明内胚层细胞层中的两个基因对芽向重力性是至关重要的。ZIG编码AtVTI11，AtVTI11是参与囊泡运输到液泡的陷阱。SGR 2与绿色荧光蛋白的融合蛋白定位于液泡和小细胞器。这些观察结果表明，ZIG和SGR2参与液泡的形成和功能，这一观点得到了电子显微镜下sgr 2和zig突变体亚细胞分析结果的支持。这些结果有力地表明，液泡参与向重力性的早期事件，SGR2和ZIG功能参与。
The endodermal cells of the shoot are thought to be the gravity-sensing cells in Arabidopsis. The amyloplasts in the endodermis that sediment in the direction of gravity may act as statoliths. Endodermis-specific expression of SGR2 and ZIG using the SCR promoter could complement the abnormal shoot gravitropism of the sgr2 and zig mutants, respectively. The abnormalities in amyloplast sedimentation observed in both mutants recovered simultaneously, These results indicate that both genes in the endodermal cell layer are crucial for shoot gravitropism. ZIG encodes AtVTI11, which is a SNARE involved in vesicle transport to the vacuole. The fusion protein of SGR2 and green fluorescent protein localized to the vacuole and small organelles. These observations indicate that ZIG and SGR2 are involved in the formation and function of the vacuole, a notion supported by the results of subcellular analysis of the sgr2 and zig mutants with electron microscopy. These results strongly suggest that the vacuole participates in the early events of gravitropism and that SGR2 and ZIG functions are involved.