Promoter-specific co-activation by Drosophila mastermind
Promoter-specific co-activation by Drosophila mastermind
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DOI：
10.1016/j.bbrc.2008.10.035
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发表时间：
2008-12-12
影响因子：
3.1
通讯作者：
Caudy, Michael A.
中科院分区：
文献类型：
--
作者：
Cave, John W.;Caudy, Michael A.
Mastermind (Mam) is a co-activator protein of binary complexes consisting of Suppressor of Hairless (Su(H)) and Notch Intracellular Domain (NICD) proteins assembled on cis-regulatory regions of target genes activated by Notch signaling. Current evidence indicates that Mastermind is necessary and sufficient for the formation of a functional Su(H)/NICD/Mam ternary complex on at least one specific architecture of Su(H) binding sites, called the SPS element (Su(H) Paired Sites). However, using transcription assays with a combination of native and synthetic Notch target gene promoters in Drosophila cultured cells, we show here that co-activation of Su(H)/NICD complexes on SPS elements by Mam is promoter-specific. Our novel results suggest this promoter specificity is mediated by additional unknown cis-regulatory elements present in the native promoters that are required for the recruitment of Mam and formation of functional Su(H)/NICD/Mam complexes on SPS elements. Together, the findings in this study suggest Mam is not always necessary and sufficient for co-activation of binary Su(H)/NICD complexes on SPS elements. (C) 2008 Elsevier Inc. All rights reserved.
