The Ca2+ signaling, Glu, and GABA responds to Cd stress in duckweed
The Ca2+ signaling, Glu, and GABA responds to Cd stress in duckweed
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Ca2 信号传导、Glu 和 GABA 对浮萍中 Cd 胁迫的响应
DOI：
10.1016/j.aquatox.2019.105352
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发表时间：
2020
影响因子：
4.5
通讯作者：
Jinsheng Sun
中科院分区：
文献类型：
--
作者：
Lin Yang;Jie Yao;Jinge Sun;Leqian Shi;Yikai Chen;Jinsheng Sun
镉（Cd）严重影响动植物健康。植物细胞中的 Ca2+ 信号对于环境胁迫的适应性反应非常重要。在这里，我们表明，50μM Cd 冲击通过将瞬时 Ca2+ 通量从 17pmol·cm-2·s-1 的流入修改为在 100μm 处从假根尖端流出 240pmol·cm-2·s-1 来刺激 Ca2+ 信号。 Ca2+信号转移到静脉和叶肉细胞。 Ca的添加减少了Cd的积累。谷氨酸受体样（GLR）蛋白的基因表达（由 Glu 激活并触发 Ca2+ 通量）在 24 小时 Cd 胁迫下显着增加。 Cd胁迫下Glu含量增加，外源Glu触发了浮萍的Ca2+信号，而Ca2+的添加对Glu含量没有影响。 GABA 是由 Glu 合成的，作为一种抑制性神经递质，经过 24 小时 Cd 处理后，其含量有所降低。在 Cd 胁迫期间，添加 GABA 会增加脱落率，而添加 Glu 会降低脱落率，这表明 Glu/GABA 比例对于响应 Cd 很重要。这项研究展示了镉胁迫期间 Glu、Ca2+、GABA 信号网络的景象。
Cadmium (Cd) affects plants and animal health seriously. Ca2+signals in plant cells are important for adaptive responses to environmental stresses. Here we showed that 50 μM Cd shock stimulated the Ca2+signal via modifying the instantaneous Ca2+flux from influx of 17 pmol·cm-2·s-1to the efflux of 240 pmol·cm-2·s-1at 100 μm from rhizoid tip. And the Ca2+signal transferred to the vein and mesophyll cell. The Ca addition decreased the accumulation of Cd. The gene expression of glutamate receptor–like (GLR) proteins, which is activated by Glu and triggers Ca2+flux, was increased significantly by 24 h Cd stress. Glu content was increased under Cd stress and exogenous Glu triggered the Ca2+signal in duckweed, while Ca2+addition caused no influence to Glu content. GABA, which is synthesized from Glu and acts as an inhibitory neurotransmitter, has been decreased with 24 h Cd treatment. GABA addition increased the abscission rate and Glu addition decreased the abscission rate during Cd stress, suggesting that the Glu/GABA ratio is important for responding to Cd. This research shows the sight of the Glu, Ca2+, GABA signaling networks during Cd stress.