hch-1, a gene required for normal hatching and normal migration of a neuroblast in C-elegans, encodes a protein related to TOLLOID and BMP-1
hch-1, a gene required for normal hatching and normal migration of a neuroblast in C-elegans, encodes a protein related to TOLLOID and BMP-1
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DOI：
10.1002/j.1460-2075.1996.tb00786.x
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发表时间：
1996-08-15
期刊：
影响因子：
11.4
通讯作者：
Katsura, I
中科院分区：
文献类型：
--
作者：
Hishida, R;Ishihara, T;Katsura, I
tolloid/骨形态发生蛋白（BMP）-1家族的蛋白质在细胞命运的分化中起重要作用。在这些蛋白质中，BMP-1在哺乳动物的软骨和骨形成中起作用，TOLLOID蛋白是果蝇胚胎的背腹轴的建立所需的，BP 10/SpAN被认为在海胆的形态发生中起作用，这些蛋白质具有一些共同的性质，首先，它们含有astacin金属蛋白酶结构域，CUB结构域和表皮生长因子样结构域，其次，它们在胚胎中表达，预期它们在细胞分化中的作用。第三，至少BMP-1和TOLLOID被认为与转化生长因子-β家族的蛋白质相互作用。本文报道秀丽隐杆线虫hch-1基因编码一个tolloid/BMP-1家族蛋白，该蛋白具有tolloid/BMP-1家族的特征结构域，与该家族的其他成员一样，在胚胎中表达，但hch-1突变体的表型表明，它是胚胎后神经母细胞正常孵化和正常迁移所必需的。此外，尽管它在胚胎发生中表达，但它不是胚胎存活所必需的。这些结果显示了tolloid/BMP-1家族蛋白的新功能，并深入了解了它们的进化。
Proteins of the tolloid/bone morphogenetic protein (BMP)-1 family play important roles in the differentiation of cell fates, Among those proteins are BMP-1, which plays a role in cartilage and bone formation in mammals, the TOLLOID protein, which is required for the establishment of the dorsoventral axis of Drosophila embryos and BP10/SpAN, which are thought to act in the morphogenesis of sea urchins, These proteins have some properties in common, First, they contain the astacin metalloprotease domain, the CUB domain and the epidermal growth factor-like domain, Second, they are expressed in embryos at stages expected for their role in cell differentiation. Third, at least BMP-1 and TOLLOID are thought to interact with proteins of the transforming growth factor-beta family. We report that the hch-1 gene of the nematode Caenorhabditis elegans encodes a tolloid/BMP-1 family protein, The protein has the characteristic domains common to the tolloid/BMP-1 family, Like other members of the family, it is expressed in embryos, However, the phenotype of hch-1 mutants shows that it is required for normal hatching and normal migration of a post-embryonic neuroblast. Furthermore, in spite of its expression in embryogenesis, it is not required for the viability of embryos, These results show new functions of the tolloid/BMP-1 family proteins and give insight into their evolution.