Stable antibacterial silver nanoparticles produced with seed-derived callus extract of Catharanthus roseus
Stable antibacterial silver nanoparticles produced with seed-derived callus extract of Catharanthus roseus
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用长春花种子愈伤组织提取物生产的稳定抗菌银纳米颗粒
DOI：
10.1080/21691401.2017.1367927
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Hideki Aoyagi
中科院分区：
文献类型：
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作者：
Dandy Ahamefula Osibe;Nneka Virginia Chiejina;Kazuyoshi Ogawa;Hideki Aoyagi
与化学产生的金属纳米颗粒相关的生物相容性和生态毒性问题已经导致人们越来越关注使用生物平台开发用于纳米颗粒合成的环境友好替代品。在此，我们报告了利用种子来源的愈伤组织ofCatharanthus roseus提取物生产稳定的银纳米颗粒（Ag NPs）。从UV-Vis光谱结果可以看出银离子的生物还原：在425 nm处观察到最大吸收，表明存在元素银。透射电子显微照片显示，银纳米颗粒分散良好，主要是球形的，粒径在2-15 nm的范围内。合成的银纳米颗粒在水性分散体中表现出胶体稳定性达120天，如UV-Vis吸收光谱和ζ电位测量所示。傅里叶变换红外光谱揭示了可能利用羟基和酰胺的还原银离子和表面稳定的银纳米粒子，分别。值得注意的是，合成的银纳米颗粒显示出相当大的抗菌作用againstEscherichia coli，即使在环境条件下储存8周后。因此，长春花愈伤组织细胞提取物可以作为一个生态友好的平台，用于合成稳定的和功能性的纳米粒子。
Biocompatibility and ecotoxicity concerns associated with chemically produced metallic nanoparticles have led to an increasing interest in the development of environmentally benign alternatives for nanoparticle synthesis using biological platforms. Herein, we report the utilization of an extract of seed-derived callus ofCatharanthus roseusfor the production of stable silver nanoparticles (Ag NPs). The bioreduction of silver ions was evident from UV–Vis spectroscopy results: the absorption maxima were observed at 425 nm, indicative of elemental silver. Transmission electron micrographs revealed that the Ag NPs were well-dispersed and predominantly spherical with particle sizes in the range of 2–15 nm. The synthesized Ag NPs exhibited colloidal stability in an aqueous dispersion for a period of 120 days, as indicated by UV–Vis absorbance spectra and zeta potential measurements. Fourier transform infrared spectroscopy revealed the possible utilization of hydroxyl groups and amides in the reduction of silver ions and surface stabilization of the Ag NPs, respectively. Notably, the synthesized Ag NPs showed considerable antibacterial action againstEscherichia colieven after 8 weeks of storage under ambient conditions. Thus, cell extracts of cultured callus ofCatharanthus roseuscould be explored as an ecofriendly platform for the synthesis of stable and functional nanoparticles.