Low CA1 spine synapse density is further reduced by castration in male non-human primates
Low CA1 spine synapse density is further reduced by castration in male non-human primates
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DOI：
10.1093/cercor/bhh012
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发表时间：
2004-05-01
期刊：
影响因子：
3.7
通讯作者：
Horvath, TL
中科院分区：
文献类型：
--
作者：
Leranth, C;Prange-Kiel, J;Horvath, TL
在非人类雌性灵长类动物中，海马体在学习和记忆中起着重要作用，其形态和功能很容易受到性腺激素的影响。我们试图确定性腺是否也影响雄性灵长类动物CA1海马区锥体细胞棘突触密度。采用无偏电子显微镜体视学方法，对正常和切除1个月龄的St Kitts vervemac(ChlorOcean Bus AtheiopsSabaeus)CA1区锥体细胞突触的体积密度、含胶质纤维酸性蛋白的胶质细胞突起的面密度和锥体细胞顶端树突的直径进行了半定量分析。去势猴棘突触的体密度(棘突突触数/微米(3))显著低于对照组(40%)，而胶质细胞突起密度(15%)显著高于对照组(15%)，位于该区域的树突直径也大于对照组(30%)。引人注目的是，与雌性相比，完整的雄性灵长类动物的脊椎突触密度低于完整或切除卵巢的雌性灵长类动物。由于灵长类动物的海马区与人类的海马区非常相似，目前的观察结果表明，在人类男性海马区的CA1层放射层中，生理水平的循环雄激素是维持正常脊椎突触密度所必需的。
The hippocampus plays a major role in learning and memory and its morphology and function are readily affected by gonadal hormones in female non-human primates. We sought to determine whether the gonads also affect pyramidal cell spine synapse density in the CA1 hippocampal area of male primates. Unbiased electron microscopic stereological calculations were performed to determine the volumetric density of pyramidal cell spine synapses and semiquantitative analyses on the surface density of glial fibrillary acidic protein-containing glia processes and the diameter of pyramidal cell apical dendrites in the CA1 area of intact and orchidectomized (1 month) St Kitts vervet monkeys (Chlorocebus aethiops sabaeus). The volumetric density (number of spine synapse/mum(3)) of spine synapses was significantly lower (40%) in the gonadectomized animals than in control monkeys; conversely, the density of glia processes was significantly higher (15%) and the diameter of dendritic shafts located in this area was also larger (30%) in the orchidectomized animals than in the controls. Strikingly, when compared to female values, intact male primates had lower spine synapse densities than either intact or ovariectomized females. Since the primate hippocampus is very similar to that of a human's, the present observations suggest that physiological levels of circulating androgen hormones are necessary to support normal spine synapse density in the CA1 stratum radiatum of human male hippocampus.