Leukocytes and the kidney contribute to interstitial inflammation in lupus nephritis
Leukocytes and the kidney contribute to interstitial inflammation in lupus nephritis
复制标题
DOI：
10.1038/sj.ki.5002625
复制
发表时间：
2008-01-01
影响因子：
19.6
通讯作者：
Koutouzov, S.
中科院分区：
文献类型：
--
作者：
Adalid-Peralta, L.;Mathian, A.;Koutouzov, S.
间质性白细胞浸润是狼疮性肾炎的一个显著特征，预示着肾功能的恶化。我们使用了两种小鼠狼疮肾炎模型，一种是慢性自发性疾病，另一种是急性干扰素α（IFN α）介导的疾病。后者的特征是几乎没有间质浸润。体内迁移试验表明，自发性肾病小鼠的脾白细胞倾向于迁移到非炎症的同系肾脏中。如果受体肾脏已经发炎，这种情况就会增强。慢性和急性肾炎小鼠的肾脏显示出类似的能力，从慢性疾病小鼠募集脾白细胞。然而，来自急性患病小鼠的白细胞未能迁移到慢性炎症的肾脏中。与慢性肾炎小鼠相比，急性肾炎小鼠肾脏炎症趋化因子CXCL 13/BLC表达减少。此外，急性肾炎小鼠的白细胞显示受损的迁移，在体外，T细胞趋化因子引诱剂。本研究将白细胞浸润与肾脏趋化因子表达和白细胞趋化性与肾脏表达的趋化因子联系起来。
Interstitial leucocyte infiltration, a prominent feature of lupus nephritis, predicts deterioration of renal function. We used two models of lupus nephritis in mice, one with chronic spontaneous disease and the other with acute interferon-alpha (IFN alpha)-mediated disease. The latter is characterized by the virtual absence of interstitial infiltration. In vivo migration assays showed that splenic leukocytes from spontaneously nephritic mice tended to migrate into non-inflamed syngeneic kidneys. This was enhanced if the recipient kidneys were already inflamed. Kidneys from both chronically and acutely nephritic mice showed similar ability to recruit splenic leukocytes from chronically diseased mice. Leukocytes from acutely diseased mice, however, failed to migrate into chronically inflamed kidney. Compared with those with chronic nephritis, the kidneys of acute nephritic mice expressed less of the inflammatory chemokine CXCL13/BLC. Moreover, leukocytes from acute nephritic mice displayed impaired migration, in vitro, to T-cell chemokine attractants. This study links leukocyte infiltration to both kidney chemokine expression, and leukocyte chemotaxis to kidney-expressed chemokines.