Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia
Molecular characterization of a new porcine rotavirus P genotype found in an asymptomatic pig in Slovenia
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DOI：
10.1016/j.virol.2006.10.001
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发表时间：
2007-03-15
期刊：
影响因子：
3.7
通讯作者：
Marin, Jozica
中科院分区：
文献类型：
--
作者：
Steyer, Andrej;Poljsak-Prijatelj, Mateja;Marin, Jozica
在斯洛文尼亚养猪场一头无症状育肥猪的粪便样本中检测到轮状病毒RNA。为了表征轮状病毒，使用 RTPCR，使用对 VP7、VP4 和 NSP4 基因具有特异性的引物。纯化特定产物并进行测序反应以对扩增基因进行分子分析。发现 VP7 基因的核苷酸和氨基酸序列与 G1 基因型菌株高度一致（85.3-88.1% 和 90.7-91.6%）。 VP7 抗原区域的系统发育和分子分析表明该样本来自 G1 基因型的新谱系。在对VP4基因的分子分析中，发现与GenBank中最相关的轮状病毒VP4基因只有70.9%的核苷酸（76.2%氨基酸）同一性。随后，还分析了 NSP4 基因。经过系统发育分析，它与NSP4 B基因型聚类，但也似乎代表了该基因型的一个新谱系。这种新的轮状病毒株被命名为 P21-5，在所有三个基因分析中与迄今为止表征的所有轮状病毒有很大不同。该菌株的毒力尚不清楚，有待研究。 (c) 2006 Elsevier Inc. 保留所有权利。
Rotaviral RNA was detected in the stool sample of an asymptomatic fattening pig at a Slovenian pig farm. To characterize the rotavirus, RTPCR was used, employing primers specific for the VP7, VP4 and NSP4 genes. Specific products were purified and the sequencing reaction was performed for the molecular analysis of amplified genes. Nucleotide and amino acid sequences of the VP7 gene were found highly identical (85.3-88.1% and 90.7-91.6%) to G1 genotype strains. Phylogenetic and molecular analyses of the VP7 antigen regions revealed the sample to be from a new lineage of G1 genotype. In the molecular analysis of the VP4 gene, only 70.9% nucleotide (76.2% amino acid) identity was found with the most related rotavirus VP4 gene from GenBank. Following this, the NSP4 gene was also analyzed. After the phylogenetic analysis, it clustered with the NSP4 B genotype, but also seemed to represent a new lineage of this genotype. This new rotavirus strain, named P21-5, differed greatly from all rotaviruses characterized so far in all three genes analyzed. The virulence of this strain is not clear yet and has to be investigated. (c) 2006 Elsevier Inc. All rights reserved.