Evidence of recent lateral gene transfer among hyperthermophilic Archaea
Evidence of recent lateral gene transfer among hyperthermophilic Archaea
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DOI：
10.1046/j.1365-2958.2000.02161.x
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发表时间：
2000-11-01
影响因子：
3.6
通讯作者：
Weiss, RB
中科院分区：
文献类型：
--
作者：
DiRuggiero, J;Dunn, D;Weiss, RB
在相邻的16 Wb区域内，两种超嗜热古细菌——furiococcus和litoralis之间共发现153个核苷酸差异。P. furiosus的16kb区域两侧是插入序列（insert sequence, is）元件，它们具有反向重复和直接重复。这两个IS元件都包含一个开放阅读框（ORF），编码一个由233个氨基酸组成的转座酶，这个16kb的区域具有典型细菌复合转座子的特征，代表了古菌之间或古菌与细菌之间基因横向转移的可能机制。在furiosus基因组序列中共发现23个同源IS元件，而在abyssi焦球菌和horikoshii焦球菌基因组中未发现全长IS元件。在海苔中只发现1个IS元素。在褐毛斑孢和褐毛斑孢中，16 kb的区域包含一个麦芽糖末端海藻糖的ABC运输系统，该系统在褐毛斑孢中具有生物化学特征。调控表达研究表明，在麦芽糖和海藻糖存在的情况下，褐毛霉和褐毛霉都能诱导位于转座子上的maIE基因和所编码的海藻糖/麦芽糖结合蛋白（TMBP）。本文讨论了作为古菌间横向基因转移机制的转位的含义。
A total of 153 nucleotide differences were found over a contiguous 16 Wb region between two hyperthermophilic Archaea, Pyrococcus furiosus and Thermococcus litoralis. The 16 kb region in P. furiosus is flanked by insertion sequence (IS) elements with inverted and direct repeats. Both IS elements contain a single open reading frame (ORF) encoding a putative protein of 233 amino acids identified as a transposase, This 16 kb region has the features of a typical bacterial composite transposon and represents a possible mechanism for lateral gene transfer between Archaea or possibly between Archaea and Bacteria. A total of 23 homologous IS elements was found in the genome sequence of P. furiosus, whereas no full-length IS elements were identified in the genomes of Pyrococcus abyssi and Pyrococcus horikoshii. Only one IS element was found in T. litoralis. In P. furiosus and T. litoralis, the 16 kb region contains an ABC transport system for maltose end trehalose that was characterized biochemically for T. litoralis. Regulation of expression studies showed that the maIE gene, located on the transposon, and the encoded trehalose/maltose-binding protein (TMBP) are induced in the presence of maltose and trehalose in both P. furiosus and T. litoralis. The implications of transposition as a mechanism for lateral gene transfer among Archaea are discussed.