A novel eukaryotic cell culture model to study antiviral activity of potential probiotic bacteria
A novel eukaryotic cell culture model to study antiviral activity of potential probiotic bacteria
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DOI：
10.1016/j.ijfoodmicro.2006.10.044
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发表时间：
2007-04-10
影响因子：
5.4
通讯作者：
Cencic, Avrelija
中科院分区：
文献类型：
--
作者：
Botic, Tanja;Klingberg, Trine Dano;Cencic, Avrelija
许多干预研究表明，益生菌可以对轮状病毒和艾滋病毒引起的腹泻起到有益的作用。尽管如此，益生菌的抗病毒作用还没有得到系统的研究。用益生菌和其他乳酸菌及其代谢产物以不同的实验设计处理非致瘤猪肠道上皮细胞(IPEC-J2)和肺泡巨噬细胞(3D4/2)。以水疱性口炎病毒(VSV)为模型病毒。抗病毒实验检测细胞存活率和病毒抑制率，免疫荧光法检测细胞存活和病毒抑制。与益生菌预先孵育细胞单层可将病毒感染力降低高达60%。所有使用的细菌都通过直接将VSV结合到它们的表面来阻止VSV与细胞单层结合。益生菌和其他乳酸菌也通过在预先处理的细胞单层中建立抗病毒状态来预防病毒感染。益生菌和其他乳酸菌在生长过程中会分泌抗病毒物质，因为当检测细菌上清液时，病毒的传染性降低了68%。在细胞培养模型中首次表明益生菌和其他乳酸菌显示出抗病毒活性。抗病毒活性的可能机制包括：1)由于细菌直接捕获病毒而阻碍VSV的吸附和细胞内化，2)在建立抗病毒保护的过程中与细胞“串扰”，以及3)产生具有直接抗病毒效果的代谢物。(C)2007 Elsevier B.V.保留所有权利。
As shown in many intervention studies, probiotic bacteria can have a beneficial effect on rotavirus and HIV-induced diarrhoea. In spite of that fact, antiviral effects of probiotic bacteria have not been systematically studied yet. Non-tumorigenic porcine intestinal epithelial cells (IPEC-J2) and alveolar macrophages (3D4/2) were treated in different experimental designs with probiotic and other lactic bacteria and their metabolic products. Vesicular stomatitis virus (VSV) was used in the study as a model virus. Cell survival and viral inhibition were determined by antiviral assay and confirmed by immunofluorescence. Pre-incubation of cell monolayers with probiotic bacteria reduced viral infectivity up to 60%. All bacteria used prevented VSV binding to the cell monolayers by direct binding of VSV to their surface. Probiotic and other lactic bacteria prevented viral infection also by establishment of the antiviral state in pre-treated cell monolayers. Probiotic and other lactic bacteria secreted antiviral substances during their growth, as the infectivity of the virus was diminished by 68% when bacterial supernatants were tested. It was shown for the first time that probiotic and other lactic bacteria exhibit an antiviral activity in a cell culture model. Possible mechanisms of antiviral activity include: 1) hindering the adsorption and cell internalisation of the VSV due to the direct trapping of the virus by the bacteria, 2) "crosstalk" with the cells in establishing the antiviral protection and 3) production of metabolites with a direct antiviral effect. (c) 2007 Elsevier B.V. All rights reserved.