Antibacterial activity of positive and negative polarity low-voltage pulsed current (LVPC) on six typical Gram-positive and Gram-negative bacterial pathogens of chronic wounds
Antibacterial activity of positive and negative polarity low-voltage pulsed current (LVPC) on six typical Gram-positive and Gram-negative bacterial pathogens of chronic wounds
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DOI：
10.1111/j.1524-475x.2007.00242.x
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发表时间：
2007-05-01
影响因子：
2.9
通讯作者：
Kramer, Axel
中科院分区：
文献类型：
--
作者：
Daeschlein, Georg;Assadian, Ojan;Kramer, Axel
电刺激（ES）对伤口愈合的积极作用已在体外和体内显示。基于血流量增加、蛋白质变性和刺激细胞防御，预期ES具有抗菌作用。虽然ES的抗菌作用已经在体外得到证实，但很少有人关注改变所施加电流的极性的直接抗菌作用。本研究旨在探讨正、负双相低压脉冲电流对慢性创面典型革兰氏阳性菌和革兰氏阴性菌的抗菌作用。使用Dermapulse（（R））-系统，针对正极性和负极性低电压脉冲电流测试三种革兰氏阴性（大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌）和三种革兰氏阳性（金黄色葡萄球菌、表皮葡萄球菌、屎肠杆菌）微生物。ES显著减少了所有供试微生物。正极性与对照组和负极性与对照组之间的降低差异显著，正极性达到最高log 10降低因子（RF）。使用正极性，测量E的最大RF。大肠杆菌（中位数log 10 RF 0.83;第25百分位数0.59，第75百分位数0.98）和最低的S.表皮（中位数log 10 RF 0.20;第25百分位数0.17，第75百分位数0.24）。然而，阳性ES对革兰氏阳性或革兰氏阴性微生物无显著差异。
The positive effect of electrical stimulation (ES) on wound healing has been shown in vitro and in vivo. On the basis of increased blood flow, protein denaturation, and stimulation of cellular defense, an antibacterial effect of ES is to be expected. Although the antibacterial effect of ES already has been demonstrated in vitro, little attention has been paid to the direct antibacterial effect of changing polarity of the applied current. The aim of this study was to investigate the antibacterial effect of positive and negative monophasic low-voltage pulsed current on typical Gram-positive and Gram-negative pathogens of chronic wounds. Using the Dermapulse((R))-System, three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae) and three Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia faecium) organisms were tested against positive and negative polarity low voltage pulsed current. All tested organisms were significantly reduced by ES. The reduction differed significantly between positive polarity and control and negative polarity and control, with the highest log10 reduction factor (RF) achieved with positive polarity. Using positive polarity, the maximum RF was measured for E. coli (median log10 RF 0.83; 25th percentile 0.59, 75th percentile 0.98) and the lowest for S. epidermidis (median log10 RF 0.20; 25th percentile 0.17, 75th percentile 0.24). Yet, there was no significant difference with positive ES against Gram-positive or Gram-negative organisms.