MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline
MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline
复制标题
DOI：
10.1016/j.devcel.2007.03.001
复制
发表时间：
2007-04-01
影响因子：
11.8
通讯作者：
Hannon, Gregory J.
中科院分区：
文献类型：
--
作者：
Carmell, Michelle A.;Girard, Angelique;Hannon, Gregory J.
小rna与Argonaute蛋白相关，并作为mRNA稳定性、高产翻译、染色质组织和基因组结构调控的序列特异性指南。在动物中，阿尔戈内特超级家族分为两个分支。Argonaute分支在RNAi和microrna介导的基因调控中与21- 22nt rna合作。Piwi分支及其26-30 nt piRNA伙伴尚未被赋予明确的功能。在小鼠中，两个piwi家族成员已被证明在精子发生中起重要作用。在这里，我们研究了破坏编码第三个家族成员MIW12的基因的影响。miwi2缺陷小鼠在减数分裂I的早期前期表现出减数分裂进展缺陷，随着年龄的增长，生殖细胞的显著和进行性损失。这些表型可能与在Miwi2突变体中检测到的转座因子的不适当激活有关。我们的观察结果表明，piwi -枝蛋白在种系转座子控制中的保守功能。
Small RNAs associate with Argonaute proteins and serve as sequence- specific guides for regulation of mRNA stability, productive translation, chromatin organization, and genome structure. In animals, the Argonaute superfamily segregates into two clades. The Argonaute clade acts in RNAi and in microRNA-mediated gene regulation in partnership with 21-22 nt RNAs. The Piwi clade, and their 26-30 nt piRNA partners, have yet to be assigned definitive functions. In mice, two Piwi-family members have been demonstrated to have essential roles in spermatogenesis. Here, we examine the effects of disrupting the gene encoding the third family member, MIW12. Miwi2-deficient mice display a meiotic-progression defect in early prophase of meiosis I and a marked and progressive loss of germ cells with age. These phenotypes may be linked to an inappropriate activation of transposable elements detected in Miwi2 mutants. Our observations suggest a conserved function for Piwi-clade proteins in the control of transposons in the germline.