Fully automated, low-cost ion chromatography system for in-situ analysis of nitrite and nitrate in natural waters
Fully automated, low-cost ion chromatography system for in-situ analysis of nitrite and nitrate in natural waters
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DOI：
10.1016/j.talanta.2020.120955
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发表时间：
2020-08-15
期刊：
影响因子：
6.1
通讯作者：
Paull, Brett
中科院分区：
文献类型：
--
作者：
Murray, Eoin;Roche, Patrick;Paull, Brett
我们开发了一种经济高效的自动化便携式 IC，用于对天然水中的亚硝酸盐和硝酸盐进行原位分析。该系统采用 3D 打印泵进行淋洗液输送，并采用基于深紫外 LED 的光学检测器。 3 分钟内即可实现亚硝酸盐和硝酸盐的等度分离和选择性检测。分析仪的总重量约为 11 公斤，包括电子设备以及用于自动分析的样品摄入系统。使用不同的样品注射环路生成线性校准范围。使用 150 μL 定量环，产生适合淡水分析的分析范围（0.05-30 mg L-1 NO2-、0.10-75 mg L-1 NO3-），而使用 10 μL 定量环，获得适合污水和生活废水分析的分析范围（0.30-100 mg L-1 NO2-、2.5-500 mg L-1 NO3-）。系统证明的色谱重复性以图形方式呈现，并且在 82 次连续运行中，峰面积和保留时间的 RSD 值小于 4%。该系统在多个地点现场部署，在不同的部署周期内分析化粪池水、废水处理厂的废水和溪流水。现场系统生成的数据与经认可的实验室仪器生成的抓取样本数据相当。
A cost-effective, automated and portable IC has been developed for in-situ analysis of nitrite and nitrate in natural waters. The system employed 3D printed pumps for eluent delivery and a deep-UV LED based optical detector. Isocratic separation and selective detection of nitrite and nitrate was achieved in under 3 min. The total weight of the analyser was similar to 11 kg, and included electronics along with a sample intake system for automated analysis. Linear calibration ranges were generated using different sample injection loops. Using a 150 mu L loop, an analytical range (0.05-30 mg L-1 NO2-, 0.10-75 mg L-1 NO3-) suitable for freshwater analysis was generated, while using a 10 mu L loop an analytical range (0.30-100 mg L-1 NO2-, 2.5-500 mg L-1 NO3-) suitable for effluent and domestic wastewater analysis was achieved. Chromatographic repeatability demonstrated by the system is graphically presented and RSD values of < 4% were obtained in terms of peak area and retention time over 82 sequential runs. The system was deployed in-situ at multiple sites for varying deployment periods analysing septic tank water, effluent from a waste water treatment plant and stream water. The data generated by the in-situ system were comparable to grab sample data generated by accredited laboratory instrumentation.