Expression of a cascading genetic network within liposomes
Expression of a cascading genetic network within liposomes
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DOI：
10.1016/j.febslet.2004.09.046
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发表时间：
2004-10-22
期刊：
影响因子：
3.5
通讯作者：
Yomo, T
中科院分区：
文献类型：
--
作者：
Ishikawa, K;Sato, K;Yomo, T
长期以来，脂质体一直被用作人工细胞的可能隔室，并已被证明可以支持各种类型的生化反应。为了提高脂质体中该系统的分子复杂程度，我们构建了一个包裹在脂质体中的两阶段遗传网络。这个两阶段遗传网络是用pTH构建的，其中第一阶段的蛋白质产物(T7RNA聚合酶)需要驱动第二阶段的蛋白质合成(GFP)。我们证明，在无细胞表达系统中构建的两阶段遗传网络在脂质体中是有效的。(C)2004年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Liposomes have long been used as possible compartments for artificial cells, and it has been shown that liposomes can sustain various types of biochemical reactions. To elevate the degree of molecular complexity of the system in liposomes, we have constructed a two-stage genetic network encapsulated in liposomes. This two-stage genetic network was constructed with the plasmid pTH, in which the protein product of the first stage (T7 RNA polymerase) is required to drive the protein synthesis of the second stage (GFP). We show that the two-stage genetic network constructed in a cell-free expression system is functional within liposomes. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.