Expression and modulation of FcεRIα and FcεRIβ in human blood basophils
Expression and modulation of FcεRIα and FcεRIβ in human blood basophils
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DOI：
10.1067/mai.2001.114653
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发表时间：
2001-05-01
影响因子：
14.2
通讯作者：
MacGlashan, DW
中科院分区：
文献类型：
--
作者：
Saini, SS;Richardson, JJ;MacGlashan, DW
背景资料：IgE受体（Fc β RI）可能以四聚体（α β γ 2）或三聚体（α γ 2）形式存在，因为Fc β RI β是Fc β RI α的膜表达载体。Fc ε RI β放大FceRI的信号传导，使得FceRI α：P化学计量的调节将影响细胞responsibility.Objective：我们检测了来自各种供体的嗜碱性粒细胞在其Fc ε RI α和Fc ε RI β蛋白表达方面的差异。在基线和IL-3培养后，通过蛋白质印迹法评估富集的血液嗜碱性粒细胞的Fc β RI α和Fc β RI β蛋白，结果：免疫印迹法检测到FceRIa亚基的2条蛋白带，分子量分别为50 kd和60 kd。60 kd条带与流式细胞术检测到的表面表达的Fc ε RI α相关（斯皮尔曼R = 0.78，P <0.01）。表面FceRIa也与Fc ε RI β蛋白相关（斯皮尔曼R = 0.92，P <0.01），Fc ε RI β蛋白水平与较高的表面Fc ε RI α表达不成比例地增加。Fc ε RI β与FceRIa的比率在供体之间变化10倍，并且与表面FceRIa相关。嗜碱性粒细胞50-kd α蛋白水平相似，尽管表面Fc β RI α表达的范围为10倍，这意味着该蛋白的储存，如在嗜酸性粒细胞中发现的那些。与嗜酸性粒细胞不同，嗜碱性粒细胞50-kd蛋白在培养中丢失，并且不存在于上清液中。P蛋白和mRNA的水平增强IL-3文化，而Fc ε RI α的表达（现在的细胞计数和60 kd）是not.Conclusion：这些研究结果表明可变的化学计量的FceRIa：P在整个细胞中，这种化学计量可以改变IL-3文化。假设所有检测到的P蛋白都是表面表达的，这些发现表明FceRIa：P的可变化学计量也与FceRIa表面表达相关。
Background: The IgE receptor (Fc epsilon RI) may exist as a tetramer (alpha beta gamma2) or a trimer (alpha gamma2) because Fc epsilon RI beta is dispensable for membrane expression of Fc epsilon RI alpha. Fc epsilon RI beta amplifies signaling of FceRI so that regulation of FceRIa: P stoichiometry would affect cellular responsiveness.Objective: We examined basophils from a variety of donors fur differences in their expression of Fc epsilon RI alpha and Fc epsilon RI beta protein.Methods: Enriched blood basophils were assessed at baseline and after IL-3 culture for Fc epsilon RI alpha and Fc epsilon RI beta protein by Western blotting, surface Fc epsilon RI alpha by flow cytometry, and Fc epsilon RI beta mRNA by real-time PCR, Basophil functional response was measured by allergen-triggered histamine release.Results: For the FceRIa subunit, 2 protein bands with molecular weights of 50 kd and 60 kd were identified by Western blots. The 60-kd band correlated to surface-expressed Fc epsilon RI alpha detected by flow cytometry (Spearman R = 0.78, P < .01). Surface FceRIa also correlated with Fc epsilon RI beta protein (Spearman R = 0.92, P < .01), Fc epsilon RI beta protein levels increased disproportionately with higher surface Fc epsilon RI alpha expression, The ratio of Fc epsilon RI beta to FceRIa varied 10-fold among donors and correlated with surface FceRIa. Basophil 50-kd a protein levels were similar despite a 10-fold range in surface Fc epsilon RI alpha expression, implying stores of this protein such as those found in eosinophils, Unlike eosinophils, the basophil 50-kd protein was lost with culture and was absent from supernatants. Levels of p protein and mRNA were enhanced by IL-3 culture, whereas Fc epsilon RI alpha expression (by now cytometry and 60 kd) was not.Conclusion: These findings demonstrate variable stoichiometry of FceRIa:P in whole cells and that this stoichiometry can be altered by IL-3 culture. With the assumption that all detected p protein is surface expressed, these findings suggest a variable stoichiometry for FceRIa:P that is also related to FceRIa surface expression.