QUANTITATIVE-ANALYSIS OF POTASSIUM CHANNEL MESSENGER-RNA EXPRESSION IN ATRIAL AND VENTRICULAR MUSCLE OF RATS
QUANTITATIVE-ANALYSIS OF POTASSIUM CHANNEL MESSENGER-RNA EXPRESSION IN ATRIAL AND VENTRICULAR MUSCLE OF RATS
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DOI：
10.1161/01.res.75.2.252
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发表时间：
1994-08-01
影响因子：
20.1
通讯作者：
MCKINNON, D
中科院分区：
文献类型：
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作者：
DIXON, JE;MCKINNON, D
用核糖核酸酶保护试验定量比较了15种不同钾通道基因在大鼠心房肌和心室肌中的表达。在这些基因中，只有Kv1.2、Kv1.4、Kv1.5、Kv2.1和Kv4.2在心肌中以显著水平表达。在心房和心室RNA样品的比较中，来自Kv1.2和Kv4.2基因的转录物显示出相对丰度的最大差异。心房肌中总Kv4亚家族mRNA的表达相对于心室肌减少约两倍，总Kv1亚家族mRNA增加70%。同时观察左心室壁钾通道mRNA表达的变化。Kv4.2在心室壁的表达有很大的梯度，心外膜肌的Kv4.2表达比乳头肌高8倍以上。其他钾通道基因在整个心室壁中以相对均匀的水平表达。结果表明，转录调控作出了显着贡献的控制钾通道在心肌中的表达和从不同区域的心肌细胞的电生理表型的变化。
The expression of 15 different potassium channel genes in rat atrial and ventricular muscle was quantitatively compared by use of an RNase protection assay. Of these genes, only five, Kv1.2, Kv1.4, Kv1.5, Kv2.1, and Kv4.2, were expressed at significant levels in cardiac muscle. In comparisons of atrial and ventricular RNA samples, transcripts from the Kv1.2 and Kv4.2 genes showed the largest differences in relative abundance. There was an approximately twofold decrease in total Kv4 subfamily mRNA expression in atrial muscle relative to ventricular muscle and a 70% increase in total Kv1 subfamily mRNA. Variation of potassium channel mRNA expression within the left ventricular wall was also examined. There was a large gradient of Kv4.2 expression across the ventricular wall, and Kv4.2 expression in epicardial muscle was more than eight times higher than in papillary muscle. Other potassium channel genes were expressed at relatively uniform levels across the ventricular wall. The results suggest that transcriptional regulation makes a significant contribution to the control of potassium channel expression in cardiac muscle and to the variation of the electrophysiological phenotype of myocytes from different regions of the myocardium.