Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection
Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection
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DOI：
10.1126/science.297.5586.1536
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发表时间：
2002-08-30
期刊：
影响因子：
56.9
通讯作者：
Mirkin, CA
中科院分区：
文献类型：
--
作者：
Cao, YWC;Jin, RC;Mirkin, CA
用寡核苷酸和拉曼活性染料标记的金纳米粒子探针对寡核苷酸靶进行了多重检测。金纳米颗粒促进银涂层的形成，所述银涂层充当染料标记的颗粒的表面增强拉曼散射促进剂，所述染料标记的颗粒已经被靶分子和微阵列格式的底层芯片捕获。该策略提供了灰度扫描检测的高灵敏度和高选择性属性，但增加了多路复用和比率能力，因为可以基于使用拉曼标签作为窄带光谱指纹的概念设计非常大量的探针。用6个拉曼标记的纳米颗粒探针区分了6个不同的DNA靶标，以及2个具有单核苷酸多态性的RNA靶标。该方法目前未优化的检测限为20飞摩尔。
Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surface-enhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of grayscale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow-band spectroscopic fingerprint. Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar.