Simultaneous release of several tachykinins and calcitonin gene-related peptide from rat spinal cord slices
Simultaneous release of several tachykinins and calcitonin gene-related peptide from rat spinal cord slices
复制标题
大鼠脊髓切片同时释放多种速激肽和降钙素基因相关肽
DOI：
10.1016/0304-3940(86)90376-9
复制
发表时间：
1986
影响因子：
2.5
通讯作者：
J. Lundberg
中科院分区：
文献类型：
--
作者：
A. Saria;R. Gamse;J. Petermann;J. Fischer;E. Theodorsson‐Norheim;J. Lundberg
Superfusion of slices of the dorsal half of rat spinal cord in vitro with 10 μM capsaicin or 60 mM potassium lead to the simultaneous release of substance P (SP)-, neurokinin A (NKA)- and calcitonin gene-related peptide (CGRP)-like immunoreactivities (LI). The ratio between capsaicin-stimulated and basal release was higher for CGRP-LI than for SP-LI, indicating that relatively more CGRP is released from sensory nerves, whereas SP is not only released from afferent neurons. High-performance liquid chromatography of NKA-LI revealed several immunoreactive components. One major peak had the retention time of synthetic NKA. A second peak eluted close to the position of synthetic eledoisin. In conclusion, capsaicin releases several bioactive peptides from sensory neurons which may mediate the acute algetic effect of chemical irritants.
