Effect of ethanol on human placental transport and metabolism of adenosine
Effect of ethanol on human placental transport and metabolism of adenosine
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DOI：
10.1016/s0143-4004(97)80038-0
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发表时间：
1997-07-01
期刊：
影响因子：
3.8
通讯作者：
Contreras, E
中科院分区：
文献类型：
--
作者：
Acevedo, CG;Huambachano, A;Contreras, E
有人认为，腺苷参与了乙醇在许多组织中的急性效应。本研究旨在评价腺苷对急性给予乙醇后灌流的离体人胎盘子叶血管反应的作用。同时探讨了乙醇对腺苷摄取和代谢的影响。采用单循环配对示踪稀释技术研究腺苷的摄取。腺苷和乙醇均引起胎盘小叶灌注压的剂量相关性增加。药理学相关浓度的乙醇（10-65 mM）显著抑制25%和50%之间的[H-3]腺苷的摄取。乙醇给药后的灌流液的薄层色谱分析显示，[3 H]腺苷代谢减少17.9 +/-0.6%。这些研究结果支持工作的假设，胎盘腺苷，至少部分地，介导的胎盘干扰引起的行政急性乙醇，这可能有助于胎儿酒精综合征的发病机制。(C)小行星1997 W. B。Saunders Company Ltd
It has been suggested that adenosine is involved in the acute effects of ethanol in a number of tissues. The present study was undertaken to evaluate the role of adenosine on the vascular responses of perfused isolated human placental cotyledons after the acute administration of ethanol. The possibility that ethanol may effect the uptake and metabolism adenosine was also investigated. Uptake of adenosine was studied using the single-circulation paired-tracer dilution technique. Both adenosine and ethanol caused a dose-related increase in perfusion pressure of placental lobules. Pharmacologically relevant concentrations of ethanol (10-65 mM) significantly inhibited the uptake of [H-3]adenosine between 25 and 50 per cent. Thin-layer chromatographic analysis of the perfusate after the administration of ethanol showed in a 17.9 +/- 0.6 per cent reduction of [3H]adenosine metabolism. These findings support the working hypothesis that placental adenosine, at least partially, mediates the placental disturbance elicited by the administration of acute ethanol, which may contribute to the pathogenesis of fetal alcohol syndrome. (C) 1997 W. B. Saunders Company Ltd.