Equilibrium binding of spin-labeled fatty acids to bovine serum albumin: suitability as surrogate ligands for natural fatty acids.
Equilibrium binding of spin-labeled fatty acids to bovine serum albumin: suitability as surrogate ligands for natural fatty acids.
复制标题
自旋标记脂肪酸与牛血清白蛋白的平衡结合：作为天然脂肪酸替代配体的适用性。
DOI：
10.1021/bi00260a023
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发表时间：
1982
期刊：
影响因子：
2.9
通讯作者：
Park,CR
中科院分区：
文献类型：
--
作者：
PerkinsJr,RC;Abumrad,N;Balasubramanian,K;Dalton,LR;Beth,AH;Park,JH;Park,CR
Ray C. Perkins，Jr.，* Nada Abumrad，K.巴拉苏布拉马尼扬湖R.作者：道尔顿作者：Jane H. Park和Charles R.公园 * 摘要：采用电子顺磁共振（EPR）和饱和转移EPR（ST-EPR）技术研究了自旋标记脂肪酸（SLFA）与牛血清白蛋白（BSA）的结合。随着SLFA与BSA的比例从约0增加到9，通过EPR光谱法估计了在C-5、C-12或C-16处标记硝酰基自由基的三种硬脂酸衍生物的结合常数。将这些值与未改性的硬脂酸酯的值进行比较。对于所有三种SLFA，很明显硝酰基残基改变了结合模式。对于SLFA：BSA比率高达1，这可能涉及BSA上对长链FA最具特异性的位点，C-16衍生物以与天然FA相似的亲和力结合。在较高的比例下，这种SLFA的缔合常数低于Mor-risett等人使用的自旋标记脂肪酸（SLFA）1。（1975）通过电子顺磁共振（EPR）光谱表征脂肪酸与血清白蛋白的结合。该技术允许区分游离和结合的SLFA，如在研究中所示，由Escherichfield等人。（1978），从而简化了关联估计
Ray C. Perkins, Jr.,* Nada Abumrad, K. Balasubramanian, L. R. Dalton, Albert H. Beth, Jane H. Park, and Charles R. Park* abstract: Electron paramagnetic resonance(EPR) and saturation transfer EPR (ST-EPR) spectroscopies were used to characterize the binding of spin-labeled fatty acid (SLFA) to bovine serum albumin (BSA). Association constants of three stearic acid derivatives labeled with a nitroxyl radical at C-5, C-12, or C-16 were estimated by EPR spectroscopy as the ratio of SLFA to BSA was increased from about 0 to 9. The values were compared to those for unmodified stearate. With all three SLFA, it was apparent that the nitroxyl residue modified the binding pattern. For SLFA: BSA ratios upto 1, which probably involves the site (s) on BSA most specific for long-chain FA, the C-16 derivative bound with an affinity similar to that of the natural FA. At higher ratios, the as-sociation constants for this SLFA were lower than those forSpin-labeled fatty acids (SLFA) 1 have been used by Mor-risett et al.(1975) to characterize fatty acid binding to serum albumin by electron paramagnetic resonance(EPR) spec-troscopy. This technique permits differentiation of free and bound SLFA, as illustrated in the study by Rehfield et al.(1978), and thusappears to simplify estimation of association