Platinum nanoflowers for surface-assisted laser desorption/ionization mass spectrometry of biomolecules
Platinum nanoflowers for surface-assisted laser desorption/ionization mass spectrometry of biomolecules
复制标题
DOI：
10.1021/jp075159d
复制
发表时间：
2007-11-08
影响因子：
3.7
通讯作者：
Arakawa, Ryuichi
中科院分区：
文献类型：
--
作者：
Kawasaki, Hideya;Yonezawa, Tetsu;Arakawa, Ryuichi
已经开发了在Pt纳米颗粒表面上具有薄突起（花瓣）的表面清洁的铂纳米材料（称为Pt纳米花）的无模板合成，并且Pt纳米花在生物分子的表面辅助激光解吸/电离质谱（SALDI-MS）上实现了上级性能。使用这种新材料，可以用较低的激光能量和较小的样品量获得高分辨率的质谱，并且不需要有机基质进行激光解吸/电离。质谱在较低的m/z区域也具有较少的障碍峰。此外，这种新的基板是相当稳定的老化，并没有表现出甜蜜点的问题，即，在整个基板表面上的相等的灵敏度。各种生物分子，包括肽和磷脂，进行了检查，和Pt nanoflower显示出良好的性能，这些材料的无基质激光解吸/电离MS。
No-template synthesis of surface clean platinum nanomaterials with thin projections (petals) on the Pt nanoparticle surface, termed Pt nanoflowers, has been developed, and Pt nanoflowers have achieved superior performance on surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) of biomolecules. With this new material, one can obtain high-resolution mass spectra with lower laser energy and smaller sample amounts, and no organic matrix is needed for laser desorption/ionization. The mass spectra also have fewer obstacle peaks at the lower m/z region. Furthermore, this novel substrate is quite stable against aging and showed no sweet-spot problems, that is, equal sensitivity on the whole substrate surface. Various biomolecules, including peptides and phospholipids, were examined, and Pt nanoflowers showed good performance for matrix-free laser desorption/ionization MS of these materials.