RNA-binding proteins and mRNA turnover in trypanosomes
RNA-binding proteins and mRNA turnover in trypanosomes
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DOI：
10.1016/s1471-4922(03)00035-7
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发表时间：
2003-04-01
影响因子：
9.6
通讯作者：
Frasch, ACC
中科院分区：
文献类型：
--
作者：
D'Orso, I;De Gaudenzi, JG;Frasch, ACC
锥虫是动质体目的原生动物寄生虫，主要通过转录后机制控制基因表达。最近发现，主要位于3'非翻译区的一些基序，如富含AU的元件（战神），可以调节mRNA的半衰期，并且能够通过与特定RNA结合蛋白的相互作用来改变体内mRNA的丰度。沿着检测到活性外泌体、去帽活性和受战神刺激的受调节的3'至5'外切核酸酶活性，这些结果表明mRNA稳定性的调节在锥虫中是必需的。这些调节过程对于不同的发育阶段是特定的，因此与允许锥虫适应可变的环境条件有关。
Trypanosomes, protozoan parasites of the order Kinetoplastida, control gene expression essentially through post-transcriptional mechanisms. Several motifs located mainly in the 3' untranslated region, such as AU-rich elements (AREs), were recently shown to modulate mRNA half-life, and are able to modify mRNA abundance in vivo through the interaction with specific RNA-binding proteins. Along with the detection of an active exosome, decapping activities and a regulated 3' to 5' exonuclease activity stimulated by AREs, these results suggest that modulation of mRNA stability is essential in trypanosomes. These regulatory processes are specific for different developmental stages and thus relevant for allowing trypanosomes to adapt to variable environmental conditions.