Isolation of three new naturally occurring compounds from the culture of Micromonospora sp P1068
Isolation of three new naturally occurring compounds from the culture of Micromonospora sp P1068
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DOI：
10.1080/14786410412331272040
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发表时间：
2005-10-01
影响因子：
2.2
通讯作者：
Timmermann, BN
中科院分区：
文献类型：
--
作者：
Gutierrez-Lugo, MT;Woldemichael, GM;Timmermann, BN
生物测定导向分离小单孢菌发酵液中的抗菌提取物。P1068分离鉴定了8个化合物，分别为(3R)3，4‘，7-三羟基异黄酮酮(1)、3-羟基脱氢大豆苷元、大豆苷元(2)、3-甲基-1H-吲哚-2-羧酸(3)、1H-吲哚-3-甲醛(4)、3-(对羟基苯基)-N-甲基丙酰胺、N-甲基苯基苯乙酰胺(5)、苯乙酸(6)、2-羟基苯乙酸(7)和4-羟基-5-甲氧基苯甲酸(8)。化合物1和5为新化合物，化合物3为首次从天然来源分离得到。评估了所有化合物对一组具有临床意义的微生物的抗菌活性。化合物4对金黄色葡萄球菌(MIC32 mU g/ml)、屎肠球菌(MIC32 mU g/m l)和大肠杆菌(MIC mU g/m l)均有抑制作用。
Bioassay guided isolation of an antibacterial extract prepared from the fermentation broth of a Micromonospora sp. P1068 led to the isolation of eight compounds identified as (3R) 3,4',7-trihydroxy-isoflavanone ( 1), 3-hydroxydehydrodaidzein, daidzein ( 2), 3-methyl-1H-indole-2- carboxylic acid ( 3), 1H- indole-3-carboxaldehyde (4), 3-(p-hydroxyphenyl)-N-methylpropionamide, N-methylphloretamide ( 5), phenyl acetic acid ( 6), 2-hydroxy phenyl acetic acid ( 7) and 4-hydroxy-5-methoxy-benzoic acid ( 8). Compounds 1 and 5 were found to be novel chemical entities while 3 was isolated from a natural source for the first time. All compounds were evaluated for their antimicrobial activities against a panel of clinically significant microorganisms. Compound 4 was active against Staphylococcus aureus ( MIC, 32 mu g/ml), Enterococcus faecium (MIC, 32 mu g/ml) and Escherichia coli (MIC, 64 mu g/ml).